Improvement of properties of Pseudomonas sp. lipase in resolution of 2-octanol

被引：0
|
作者：
Wang, Z
Quan, J
Weng, L
Zheng, LY
Liu, N
Dong, H
Cao, SG [1 ]
机构：
[1] Jilin Univ, Key Lab Mol Enzymol & Engn, Educ Minist, Changchun 130023, Peoples R China
[2] Jilin Univ, Coll Life Sci, Changchun 130023, Peoples R China
来源：
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES | 2003年 / 19卷 / 04期
关键词：
lipase; surfactant; salt hydrate pair; esterification; enantioselectivity;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A novel preparation method was developed to form a surfactant-lipase complex for the resolution of 2-octanol in a solvent-free system. The E value was improved from 6.64 to 120.2. The lipase modified by the anionic surfactant possessed a low solubility in the solvent-free system, which was beneficial to the recovery and the repeated usage of the lipase. The surfactant-lipase complex maintained a high enantioselectivity after five cycles of usage. The effect of water on both the activity and the enantioselectivity of the lipase has also been investigated; the direct addition of a salt hydrate pair of Na4P2O7. H2O and Na4P2O7 can dramatically activate the modified enzyme.
引用
收藏
页码：446 / 449
页数：4
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