Three-photon light-sheet fluorescence microscopy

被引：40

作者：

Escobet-Montalban, Adria ^{[1
]}

Gasparoli, Federico M. ^{[1
]}

Nylk, Jonathan ^{[1
]}

Liu, Pengfei ^{[1
]}

Yang, Zhengyi ^{[1
,2
]}

Dholakia, Kishan ^{[1
]}

机构：

[1] Univ St Andrews, Sch Phys & Astron, Scottish Univ Phys Alliance, North Haugh KY16 9SS, Fife, Scotland

[2] Diamond Light Source, Electron Bioimaging Ctr, Harwell Sci & Innovat Campus, Didcot OX11 0DE, Oxon, England

来源：

OPTICS LETTERS | 2018年 / 43卷 / 21期

基金：

英国工程与自然科学研究理事会; 欧盟地平线“2020”;

关键词：

DEEP; EMBRYOS;

D O I：

10.1364/OL.43.005484

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We present the first demonstration of three-photon excitation light-sheet fluorescence microscopy. Light-sheet fluorescence microscopy in single- and two-photon modes has emerged as a powerful wide-field, low-photodamage technique for Fast volumetric imaging of biological samples. We extend this imaging modality to the three-photon regime, enhancing its penetration depth. Our present study uses a conventional femtosecond pulsed laser at 1000 nm wavelength for the imaging of 450 mu m diameter cellular spheroids. In addition, we show, experimentally and through numerical simulations, the potential advantages in three-photon light-sheet microscopy of using propagation-invariant Bessel beams in preference to Gaussian beams. (C) 2018 Optical Society of America

引用

页码：5484 / 5487

页数：4

共 50 条

[31] Multiscale imaging of plant development by light-sheet fluorescence microscopy
Ovecka, Miroslav
von Wangenheim, Daniel
Tomancak, Pavel
Samajova, Olga
Komis, George
Samaj, Jozef
[J]. NATURE PLANTS, 2018, 4 (09) : 639 - 650
[32] Removing striping artifacts in light-sheet fluorescence microscopy: a review
Ricci, Pietro
Gavryusev, Vladislav
Mullenbroich, Caroline
Turrini, Lapo
de Vito, Giuseppe
Silvestri, Ludovico
Sancataldo, Giuseppe
Pavone, Francesco Saverio
[J]. PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 2022, 168 : 52 - 65
[33] Multiscale imaging of plant development by light-sheet fluorescence microscopy
Miroslav Ovečka
Daniel von Wangenheim
Pavel Tomančák
Olga Šamajová
George Komis
Jozef Šamaj
[J]. Nature Plants, 2018, 4 : 639 - 650
[34] Illuminating the Unseen: Data at Depth with Light-Sheet Fluorescence Microscopy
Kugler, Elisabeth
Wachsmuth, Malte
Kromm, Dimitri
[J]. Biophotonics International, 2023, 30 (06): : 28 - 33
[35] Light-sheet optimization for microscopy
Wilding, Dean
Pozzi, Paolo
Soloviev, Oleg
Vdovin, Gleb
Verhaegen, Michel
[J]. ADAPTIVE OPTICS AND WAVEFRONT CONTROL FOR BIOLOGICAL SYSTEMS II, 2016, 9717
[36] Light-sheet microscopy on AutoPilot
[J]. Nature Methods, 2017, 14 : 11 - 11
[37] Subvoxel light-sheet microscopy
Oliver Graydon
[J]. Nature Photonics, 2019, 13 : 139 - 139
[38] The light-sheet microscopy revolution
Girkin, J. M.
Carvalho, M. T.
[J]. JOURNAL OF OPTICS, 2018, 20 (05)
[39] Subvoxel light-sheet microscopy
Graydon, Oliver
[J]. NATURE PHOTONICS, 2019, 13 (03) : 139 - 139
[40] Light-Sheet Microscopy in Neuroscience
Hillman, Elizabeth M. C.
Voleti, Venkatakaushik
Li, Wenze
Yu, Hang
[J]. ANNUAL REVIEW OF NEUROSCIENCE, VOL 42, 2019, 42 : 295 - +

← 1 2 3 4 5 →