Human Antibody Repertoire following Ebola Virus Infection and Vaccination

被引：11
|
作者：
Fuentes, Sandra [1 ]
Ravichandran, Supriya [1 ]
Coyle, Elizabeth M. [1 ]
Klenow, Laura [1 ]
Khurana, Surender [1 ]
机构：
[1] US FDA, Ctr Biol Evaluat & Res, Div Viral Prod, 10903 New Hampshire Ave, Silver Spring, MD 20993 USA
来源：
ISCIENCE | 2020年 / 23卷 / 03期
关键词：
PROTECTS NONHUMAN-PRIMATES; VACCINES; TRANSMISSION; CHALLENGE; TRIAL; ASSAY;
D O I：
10.1016/j.isci.2020.100920
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Limited knowledge exists on the quality of polyclonal antibody response generated following Ebola virus (EBOV) infection compared with vaccination. Polyclonal antibody repertoire in plasma following EBOV infection in survivors was compared with ChAd3-MVA prime-boost human vaccination. Higher antibody binding and affinity to GP was observed in survivors compared with vaccinated plasma that correlated with EBOV neutralization. Surprisingly, a predominant IgM response was generated after prime-boost vaccination, whereas survivors demonstrated IgG-dominant antibody response. EBOV infection induced more diverse antibody epitope repertoire compared with vaccination. A strong binding to antigenic sites in the fusion peptide and another in the highly conserved GP2-HR2 domain was preferentially recognized by EBOV survivors than vaccinated individuals that correlated strongly with EBOV neutralization titers. These findings will help development and evaluation of effective Ebola countermeasures including therapeutics and vaccines.
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页数：24
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