Human Antibody Repertoire following Ebola Virus Infection and Vaccination

被引：11
|
作者：
Fuentes, Sandra [1 ]
Ravichandran, Supriya [1 ]
Coyle, Elizabeth M. [1 ]
Klenow, Laura [1 ]
Khurana, Surender [1 ]
机构：
[1] US FDA, Ctr Biol Evaluat & Res, Div Viral Prod, 10903 New Hampshire Ave, Silver Spring, MD 20993 USA
来源：
ISCIENCE | 2020年 / 23卷 / 03期
关键词：
PROTECTS NONHUMAN-PRIMATES; VACCINES; TRANSMISSION; CHALLENGE; TRIAL; ASSAY;
D O I：
10.1016/j.isci.2020.100920
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Limited knowledge exists on the quality of polyclonal antibody response generated following Ebola virus (EBOV) infection compared with vaccination. Polyclonal antibody repertoire in plasma following EBOV infection in survivors was compared with ChAd3-MVA prime-boost human vaccination. Higher antibody binding and affinity to GP was observed in survivors compared with vaccinated plasma that correlated with EBOV neutralization. Surprisingly, a predominant IgM response was generated after prime-boost vaccination, whereas survivors demonstrated IgG-dominant antibody response. EBOV infection induced more diverse antibody epitope repertoire compared with vaccination. A strong binding to antigenic sites in the fusion peptide and another in the highly conserved GP2-HR2 domain was preferentially recognized by EBOV survivors than vaccinated individuals that correlated strongly with EBOV neutralization titers. These findings will help development and evaluation of effective Ebola countermeasures including therapeutics and vaccines.
引用
收藏
页数：24
相关论文
共 50 条
  • [21] Antibody-Dependent Enhancement of Ebola Virus Infection by Human Antibodies Isolated from Survivors
    Kuzmina, Natalia A.
    Younan, Patrick
    Gilchuk, Pavlo
    Santos, Rodrigo, I
    Flyak, Andrew, I
    Ilinykh, Philipp A.
    Huang, Kai
    Lubaki, Ndongala M.
    Ramanathan, Palaniappan
    Crowe, James E., Jr.
    Bukreyev, Alexander
    CELL REPORTS, 2018, 24 (07): : 1802 - +
  • [22] Advances in Ebola virus vaccination
    Clarke, Elizabeth C.
    Bradfute, Steven B.
    LANCET INFECTIOUS DISEASES, 2017, 17 (08): : 787 - 788
  • [23] THE NATURE OF THE AUTOIMMUNE ANTIBODY REPERTOIRE IN HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 INFECTION
    DITZEL, HJ
    BARBAS, SM
    BARBAS, CF
    BURTON, DR
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (09) : 3710 - 3714
  • [24] High Throughput Sequencing of the Human Antibody Repertoire in Response to Influenza Vaccination
    Jiang, Ning
    He, Jiankui
    Weinstein, Joshua
    Penland, Lolita
    Saaki, Sanae
    He, Xiaosong
    Dekker, Cornelia
    Wilson, Patrick
    Greenberg, Harry
    Davis, Mark
    Fisher, Daniel
    Quake, Stephen
    JOURNAL OF IMMUNOLOGY, 2012, 188
  • [25] Infection by the Ebola virus
    Roca, B
    Simon, E
    MEDICINA CLINICA, 1996, 107 (07): : 255 - 256
  • [26] Ebola Virus Infection
    Bozkurt, Ilkay
    Leblebicioglu, Hakan
    MEDITERRANEAN JOURNAL OF INFECTION MICROBES AND ANTIMICROBIALS, 2014, 3
  • [27] The ongoing evolution of antibody-based treatments for Ebola virus infection
    Mendoza, Emelissa J.
    Racine, Trina
    Kobinger, Gary P.
    IMMUNOTHERAPY, 2017, 9 (05) : 435 - 450
  • [28] Effects of antibody on viral kinetics in simian/human immunodeficiency virus infection: Implications for vaccination
    Zhang, L
    Ribeiro, RM
    Mascola, JR
    Lewis, MG
    Stiegler, G
    Katinger, H
    Perelson, AS
    Davenport, MP
    JOURNAL OF VIROLOGY, 2004, 78 (10) : 5520 - 5522
  • [29] Monoclonal antibody prophylaxis protects against lethal Ebola virus infection
    James, Rebekah
    Stonier, Spencer W.
    Bakken, Russell
    Bornholdt, Zachary A.
    Dye, John M.
    JOURNAL OF IMMUNOLOGY, 2018, 200 (01):
  • [30] Epitopes required for antibody-dependent enhancement of Ebola virus infection
    Takada, Ayato
    Ebihara, Hideki
    Feldmann, Heinz
    Geisbert, Thomas W.
    Kawaoka, Yoshihiro
    JOURNAL OF INFECTIOUS DISEASES, 2007, 196 : S347 - S356
12345