Triacylglycerol analysis of pequi (Caryocar brasiliensis Camb.) oil by electrospray and tandem mass spectrometry

Electrospray ionization liquid chromatography/tandem mass spectrometry was used to identify the fatty acid and triacylglycerol (TAG) composition of pequi (Caryocar brasiliensis Camb.) oil. The fatty acid composition was relatively simple; the oil contained approximately 442 and 517 g kg-(1) of palmitic and oleic acids respectively. Linoleic and stearic acids were found in much smaller amounts. The TAG composition of pequi oil is also relatively simple with trioleoyl glycerol (OOO, 56 g kg-(1)), palmitoyl dioleoyl glycerol (POO, 466 g kg-(1)) and dipalmitoyl oleoyl glycerol (POP, 452 g kg-(1)) comprising 974 g kg-(1) of the total. Dioleoyl stearoyl glycerol (OOS) was found in small amounts (5.2 g kg-(1)). All TAGs were identified via sodium adduct molecular ions [M + Na](+), where M is the TAG in question. Tandem mass analysis provided a very useful fragmentation pattern showing both sodium adduct diacylglycerol ions and diacylglycerol ions with the neutral loss of fatty acid residues. (c) 2005 Society of Chemical Industry.