Rapid Natural-Abundance 2D 13C-13C Correlation Spectroscopy Using Dynamic Nuclear Polarization Enhanced Solid-State NMR and Matrix-Free Sample Preparation

被引：151
|
作者：
Takahashi, Hiroki [1 ,2 ]
Lee, Daniel [1 ,2 ]
Dubois, Lionel [1 ,2 ]
Bardet, Michel [1 ,2 ]
Hediger, Sabine [1 ,2 ]
De Paepe, Gael [1 ,2 ]
机构：
[1] UMR E3 CEA UJF, Lab Chim Inorgan & Biol, F-38054 Grenoble, France
[2] Inst Nanosci & Cryogenie CEA DSM, CNRS, F-38054 Grenoble, France
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 47期
关键词：
2D correlation; analytical methods; dynamic nuclear polarization; natural abundance; NMR spectroscopy; RESONANCE; MEMBRANES; PROTEINS;
D O I：
10.1002/anie.201206102
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Ultra-fast: Substantial solid-state NMR sensitivity can be achieved using dynamic nuclear polarization and matrix-free sample preparation (that is, absence of solvent and cryoprotectant). This method avoids line-broadening, maximizes sample filling factor, and allows natural-abundance 2D 13C-13C dipolar correlation experiments to be carried out in tens of minutes. MW=microwaves. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：11766 / 11769
页数：4
相关论文
共 50 条
  • [31] Quantitative Structural Constraints for Organic Powders at Natural Isotopic Abundance Using Dynamic Nuclear Polarization Solid-State NMR Spectroscopy
    Mollica, Giulia
    Dekhil, Myriam
    Ziarelli, Fabio
    Thureau, Pierre
    Viel, Stephane
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2015, 54 (20) : 6028 - 6031
  • [32] Assessing the extent of decomposition of natural organic materials using solid-state C-13 NMR spectroscopy
    Baldock, JA
    Oades, JM
    Nelson, PN
    Skene, TM
    Golchin, A
    Clarke, P
    AUSTRALIAN JOURNAL OF SOIL RESEARCH, 1997, 35 (05): : 1061 - 1083
  • [33] EXTENSIVE H-1-NMR RESONANCE ASSIGNMENT OF PROTEINS USING NATURAL-ABUNDANCE GRADIENT-ENHANCED 13C-1H CORRELATION SPECTROSCOPY
    MEDVEDEVA, S
    SIMORRE, JP
    BRUTSCHER, B
    GUERLESQUIN, F
    MARION, D
    FEBS LETTERS, 1993, 333 (03): : 251 - 256
  • [34] J-deconvolution using maximum entropy reconstruction applied to 13C-13C solid-state cross-polarization magic-angle-spinning NMR of proteins
    Scholz, Ingo
    Jehle, Stefan
    Schmieder, Peter
    Hiller, Matthias
    Eisenmenger, Frank
    Oschkinat, Hartmut
    Van, Rossum, Barth-Jan
    Journal of the American Chemical Society, 2007, 129 (21): : 6682 - 6683
  • [35] EXPERIMENTAL SETUP FOR ENHANCED C-13 NMR-SPECTROSCOPY IN SOLIDS USING DYNAMIC NUCLEAR-POLARIZATION
    WIND, RA
    ANTHONIO, FE
    DUIJVESTIJN, MJ
    SMIDT, J
    TROMMEL, J
    DEVETTE, GMC
    JOURNAL OF MAGNETIC RESONANCE, 1983, 52 (03) : 424 - 434
  • [36] J-Deconvolution using maximum entropy reconstruction applied to 13C-13C solid-state cross-polarization magic-angle-spinning NMR of proteins
    Scholz, Ingo
    Jehle, Stefan
    Schmieder, Peter
    Hiller, Matthias
    Eisenmenger, Frank
    Oschkinat, Hartmut
    van Rossum, Barth-Jan
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (21) : 6682 - +
  • [37] STRUCTURAL AND DYNAMIC PROPERTIES OF SOIL ORGANIC-MATTER AS REFLECTED BY C-13 NATURAL-ABUNDANCE, PYROLYSIS MASS-SPECTROMETRY AND SOLID-STATE C-13 NMR-SPECTROSCOPY IN DENSITY FRACTIONS OF AN OXISOL UNDER FOREST AND PASTURE
    GOLCHIN, A
    OADES, JM
    SKJEMSTAD, JO
    CLARKE, P
    AUSTRALIAN JOURNAL OF SOIL RESEARCH, 1995, 33 (01): : 59 - 76
  • [38] Photochemically induced dynamic nuclear polarization in bacterial photosynthetic reaction centres observed by 13C solid-state NMR
    Matysik, J
    Alia
    Gast, P
    Lugtenburg, J
    Hoff, AJ
    de Groot, HJM
    PERSPECTIVES ON SOLID STATE NMR IN BIOLOGY, 2001, 1 : 215 - 225
  • [39] Structural characterization of 13C-enriched humins and alkali-treated 13C humins by 2D solid-state NMR
    van Zandvoort, Ilona
    Koers, Eline J.
    Weingarth, Markus
    Bruijnincx, Pieter C. A.
    Baldus, Marc
    Weckhuysen, Bert M.
    GREEN CHEMISTRY, 2015, 17 (08) : 4383 - 4392
  • [40] Identifying inter-residue resonances in crowded 2D 13C–13C chemical shift correlation spectra of membrane proteins by solid-state MAS NMR difference spectroscopy
    Yimin Miao
    Timothy A. Cross
    Riqiang Fu
    Journal of Biomolecular NMR, 2013, 56 : 265 - 273
12345