Rapid Natural-Abundance 2D 13C-13C Correlation Spectroscopy Using Dynamic Nuclear Polarization Enhanced Solid-State NMR and Matrix-Free Sample Preparation

被引：151

作者：

Takahashi, Hiroki ^{[1
,2
]}

Lee, Daniel ^{[1
,2
]}

Dubois, Lionel ^{[1
,2
]}

Bardet, Michel ^{[1
,2
]}

Hediger, Sabine ^{[1
,2
]}

De Paepe, Gael ^{[1
,2
]}

机构：

[1] UMR E3 CEA UJF, Lab Chim Inorgan & Biol, F-38054 Grenoble, France

[2] Inst Nanosci & Cryogenie CEA DSM, CNRS, F-38054 Grenoble, France

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2012年 / 51卷 / 47期

关键词：

2D correlation; analytical methods; dynamic nuclear polarization; natural abundance; NMR spectroscopy; RESONANCE; MEMBRANES; PROTEINS;

D O I：

10.1002/anie.201206102

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Ultra-fast: Substantial solid-state NMR sensitivity can be achieved using dynamic nuclear polarization and matrix-free sample preparation (that is, absence of solvent and cryoprotectant). This method avoids line-broadening, maximizes sample filling factor, and allows natural-abundance 2D 13C-13C dipolar correlation experiments to be carried out in tens of minutes. MW=microwaves. Copyright © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

引用

页码：11766 / 11769

页数：4

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