A highly specific ELISA for diagnosis of 2009 influenza A (H1N1) virus infections

被引：11
|
作者：
Lu, Chun-Yi [1 ]
Chang, Luan-Yin [1 ]
Chen, Pei-Jer [2 ]
Xia, Ning-Shao [3 ]
Shao, Pei-Lan [1 ]
Huang, Li-Min [1 ]
机构：
[1] Natl Taiwan Univ, Dept Pediat, Coll Med, Natl Taiwan Univ Hosp, Taipei 100, Taiwan
[2] Natl Taiwan Univ, Dept Internal Med, Natl Taiwan Univ Hosp, Coll Med, Taipei 100, Taiwan
[3] Xiamen Univ, Natl Inst Diagnost & Vaccine Dev Infect Dis, Minist Educ Cell Biol & Tumour Cell Engn, Key Lab, Xiamen, Peoples R China
来源：
JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION | 2012年 / 111卷 / 12期
关键词：
diagnosis; ELISA; pandemic; 2009 Influenza A (H1N1); TESTS; IMMUNOASSAY; SENSITIVITY; PCR;
D O I：
10.1016/j.jfma.2011.11.029
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Background/Purpose: Accurate and timely diagnosis is vital for the clinical management of influenza. A 2009 pandemic influenza A (H1N1)-specific enzyme-linked immunosorbent assay (ELISA) was tested with selected clinical samples. Methods: A selection of 90 throat swab samples with various viral loads of 2009 pandemic influenza A (H1N1) were tested. Results: Using the reverse transcriptase polymerase chain reaction as a gold standard, the overall sensitivity (0.57) was higher than that of the QuickVue Influenza A+B Test (0.43). The specificity of the ELISA was 1.0 using the selected sample set. The positive and negative predictive values were 1 and 0.4, respectively. Conclusion: The ELISA is an easy to perform, highly specific, and fairly sensitive diagnostic tool for the 2009 pandemic influenza A (H1N1) virus infections. A strong correlation was found between viral load and specificity. Copyright (C) 2012, Elsevier Taiwan LLC & Formosan Medical Association. All rights reserved.
引用
收藏
页码：693 / 697
页数：5
相关论文
共 50 条
  • [41] Myocarditis associated with 2009 influenza A (H1N1) virus in children
    Baruteau, Alban-Elouen
    Boimond, Nicolas
    Ramful, Duksha
    CARDIOLOGY IN THE YOUNG, 2010, 20 (03) : 351 - +
  • [42] The Seroprevalence of Pandemic Influenza H1N1 (2009) Virus in China
    Xu, Cuiling
    Bai, Tian
    Iuliano, A. Danielle
    Wang, Min
    Yang, Lei
    Wen, Leying
    Zeng, Yuhong
    Li, Xiaodan
    Chen, Tao
    Wang, Wei
    Hu, Ying
    Yang, Limei
    Li, Zi
    Zou, Shumei
    Li, Dexin
    Wang, Shiwen
    Feng, Zijian
    Zhang, Yanping
    Yu, Hongjie
    Yang, Weizhong
    Wang, Yu
    Widdowson, Marc-Alain
    Shu, Yuelong
    PLOS ONE, 2011, 6 (04):
  • [43] LABORATORY DIAGNOSIS OF PANDEMIC INFLUENZA A(H1N1) 2009 INFECTION
    Ciblak, Meral Akcay
    Bozkaya, Emel
    JOURNAL OF ISTANBUL FACULTY OF MEDICINE-ISTANBUL TIP FAKULTESI DERGISI, 2010, 73 (00): : 27 - 33
  • [44] THE USE OF ANTIVIRALS FOR 2009 H1N1 INFLUENZA VIRUS INFECTION
    Norwood, Ann
    Courtney, Brooke
    Toner, Eric
    Adalja, Amesh
    BIOSECURITY AND BIOTERRORISM-BIODEFENSE STRATEGY PRACTICE AND SCIENCE, 2009, 7 (02) : 217 - 220
  • [45] Pitfalls in Diagnosis of Pandemic (Novel) A/H1N1 2009 Influenza
    Singh, Kamaljit
    Vasoo, Shawn
    Stevens, Jane
    Schreckenberger, Paul
    Trenholme, Gordon
    JOURNAL OF CLINICAL MICROBIOLOGY, 2010, 48 (04) : 1501 - 1503
  • [46] Descriptive clinical and epidemiological characteristics of influenza A H1N1 2009 virus infections in pigs in England
    Williamson, S. M.
    Tucker, A. W.
    McCrone, I. S.
    Bidewell, C. A.
    Brons, N.
    Habernoll, H.
    Essen, S. C.
    Brown, I. H.
    Wood, J. L. N.
    VETERINARY RECORD, 2012, 171 (11) : 271 - U47
  • [47] Virus-free ELISA method for the determination of influenza A/H1N1/2009 antibodies in human serum
    Lopez-Pacheco, Felipe
    Mendoza-Ochoa, Gonzalo I.
    Freiden, Pamela
    Oropeza-Almazan, Yuriana
    Shultz-Cherry, Stacey
    Guerra, Manuel I. Zertuche
    Bulnes-Abundis, David
    Elizondo-Montemayor, Leticia
    Hernandez-Torre, Martin
    Moises Alvarez, Mario
    INFLUENZA AND OTHER RESPIRATORY VIRUSES, 2011, 5 : 135 - 138
  • [48] Infections with A(H1N1)2009 Influenza Virus in Poland During the Last Pandemic: Experience of the National Influenza Center
    Romanowska, M.
    Stefanska, I.
    Donevski, S.
    Brydak, L. B.
    RESPIRATORY REGULATION - THE MOLECULAR APPROACH, 2013, 756 : 271 - 283
  • [49] Influenza A H1N1 virus in Indian pigs & its genetic relatedness with pandemic human influenza A 2009 H1N1
    Nagarajan, K.
    Saikumar, G.
    Arya, Rahul Singh
    Gupta, Ashish
    Somvanshi, R.
    Pattnaik, B.
    INDIAN JOURNAL OF MEDICAL RESEARCH, 2010, 132 (02) : 160 - 167
  • [50] Estimation of Seroprevalence of the Pandemic H1N1 2009 Influenza Virus Using a Novel Virus-Free ELISA Assay for the Detection of Specific Antibodies
    Mavrouli, Maria D.
    Routsias, John G.
    Maltezou, Helena C.
    Spanakis, Nicholas
    Tsakris, Athanassios
    VIRAL IMMUNOLOGY, 2011, 24 (03) : 221 - 226
12345