A highly specific ELISA for diagnosis of 2009 influenza A (H1N1) virus infections

被引：11
|
作者：
Lu, Chun-Yi [1 ]
Chang, Luan-Yin [1 ]
Chen, Pei-Jer [2 ]
Xia, Ning-Shao [3 ]
Shao, Pei-Lan [1 ]
Huang, Li-Min [1 ]
机构：
[1] Natl Taiwan Univ, Dept Pediat, Coll Med, Natl Taiwan Univ Hosp, Taipei 100, Taiwan
[2] Natl Taiwan Univ, Dept Internal Med, Natl Taiwan Univ Hosp, Coll Med, Taipei 100, Taiwan
[3] Xiamen Univ, Natl Inst Diagnost & Vaccine Dev Infect Dis, Minist Educ Cell Biol & Tumour Cell Engn, Key Lab, Xiamen, Peoples R China
来源：
JOURNAL OF THE FORMOSAN MEDICAL ASSOCIATION | 2012年 / 111卷 / 12期
关键词：
diagnosis; ELISA; pandemic; 2009 Influenza A (H1N1); TESTS; IMMUNOASSAY; SENSITIVITY; PCR;
D O I：
10.1016/j.jfma.2011.11.029
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Background/Purpose: Accurate and timely diagnosis is vital for the clinical management of influenza. A 2009 pandemic influenza A (H1N1)-specific enzyme-linked immunosorbent assay (ELISA) was tested with selected clinical samples. Methods: A selection of 90 throat swab samples with various viral loads of 2009 pandemic influenza A (H1N1) were tested. Results: Using the reverse transcriptase polymerase chain reaction as a gold standard, the overall sensitivity (0.57) was higher than that of the QuickVue Influenza A+B Test (0.43). The specificity of the ELISA was 1.0 using the selected sample set. The positive and negative predictive values were 1 and 0.4, respectively. Conclusion: The ELISA is an easy to perform, highly specific, and fairly sensitive diagnostic tool for the 2009 pandemic influenza A (H1N1) virus infections. A strong correlation was found between viral load and specificity. Copyright (C) 2012, Elsevier Taiwan LLC & Formosan Medical Association. All rights reserved.
引用
收藏
页码：693 / 697
页数：5
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