HPAEC-PAD method for the analysis of alkaline hydrolyzates of Haemophilus influenzae type b capsular polysaccharide

被引：12

作者：

de Haan, Alex ^{[1
]}

van der Put, Robert M. F. ^{[1
]}

Beurret, Michel ^{[1
]}

机构：

[1] RIVM, Natl Inst Publ Hlth & Environm, Unit Vaccinol, Ctr Infect Dis Control CIb, NL-3720 BA Bilthoven, Netherlands

来源：

BIOMEDICAL CHROMATOGRAPHY | 2013年 / 27卷 / 09期

关键词：

Haemophilus influenzae type b (Hib); polysaccharide analysis; conjugate vaccine analysis; high performance anion exchange chromatography (HPAEC); pulsed amperometric detection (PAD); alkaline hydrolysis; ANION-EXCHANGE CHROMATOGRAPHY; PULSED-AMPEROMETRIC DETECTION; TOXOID-CONJUGATE VACCINE; HEMOPHILUS-INFLUENZAE; QUANTIFICATION; COMBINATION; SACCHARIDE; STABILITY; ASSAY; PRP;

D O I：

10.1002/bmc.2918

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A gradient method has been devised for the rapid analysis of alkaline hydrolyzates of Haemophilus influenzae type b (Hib) capsular polysaccharide-based vaccines by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). As compared with published procedures, peak shape and sensitivity were significantly improved with this approach, analysis time was short and there was little interference from impurities. The limits of detection and quantification were established with a purified reference polysaccharide. We propose this method as a practical alternative for the analysis of minute amounts of Hib polysaccharide, which can be lower than with the conventional approaches. Copyright (C) 2013 John Wiley & Sons, Ltd.

引用

页码：1137 / 1142

页数：6

共 50 条

[1] HPAEC-PAD quantification of Haemophilus influenzae type b polysaccharide in upstream and downstream samples
van der Put, Robert M. F.
de Haan, Alex
van den Ijssel, Jan G. M.
Hamidi, Ahd
Beurret, Michel
VACCINE, 2015, 33 (48) : 6908 - 6913
[2] Uncover aldehydes in biomass hydrolyzates: disproportionation of aldehydes in alkaline solution and subsequent measurement using an automated HPAEC-PAD method
Nico Anders
Maike van Ohlen
Tim Jestel
Laura Herbst
Mohamed Amine Jmel
Issam Smaali
Antje C. Spiess
Analytical and Bioanalytical Chemistry, 2020, 412 : 5593 - 5600
[3] Uncover aldehydes in biomass hydrolyzates: disproportionation of aldehydes in alkaline solution and subsequent measurement using an automated HPAEC-PAD method
Anders, Nico
van Ohlen, Maike
Jestel, Tim
Herbst, Laura
Jmel, Mohamed Amine
Smaali, Issam
Spiess, Antje C.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2020, 412 (23) : 5593 - 5600
[4] CAPSULAR POLYSACCHARIDE OF HAEMOPHILUS-INFLUENZAE TYPE-B AS A VACCINE
PARKE, JC
PEDIATRIC INFECTIOUS DISEASE JOURNAL, 1987, 6 (08) : 795 - 798
[5] Molecular size distribution assessment of Haemophilus influenzae vaccine containing lactose by HPAEC-PAD and colorimetric assays
Nadim, Ahmed H.
Hussein, Asmaa R.
Rezk, Mamdouh R.
Fathalla, Faten Abdel Aziz
El-Saharty, Yasser S.
ANALYTICAL BIOCHEMISTRY, 2022, 653
[6] Study of the chemical stability of the capsular polysaccharide produced by Haemophilus influenzae type b
Cintra, Felipe de Oliveira
Takagi, Mickie
CARBOHYDRATE POLYMERS, 2015, 116 : 167 - 172
[7] MUCOSAL ANTIBODIES TO HAEMOPHILUS-INFLUENZAE TYPE-B CAPSULAR POLYSACCHARIDE
GILSDORF, JR
MCDONNELL, WM
PEDIATRIC RESEARCH, 1991, 29 (05) : 420 - 423
[8] MOLECULAR-SIZE ANALYSIS OF HAEMOPHILUS-INFLUENZAE TYPE-B CAPSULAR POLYSACCHARIDE
HENNESSEY, JP
BEDNAR, B
MANAM, V
JOURNAL OF LIQUID CHROMATOGRAPHY, 1993, 16 (08): : 1715 - 1729
[9] MATERNAL IMMUNIZATION WITH THE CAPSULAR POLYSACCHARIDE VACCINE FOR HAEMOPHILUS-INFLUENZAE TYPE-B
GLEZEN, WP
ENGLUND, JA
SIBER, GR
SIX, HR
TURNER, C
SHRIVER, D
HINKLEY, CM
FALCAO, O
JOURNAL OF INFECTIOUS DISEASES, 1992, 165 : S134 - S136
[10] Improving the yield of released capsular polysaccharide from Haemophilus influenzae type b.
Licari, PJ
Merritt, J
Allard, GW
Nesman, CJ
O'Toole, LP
Rost, BE
Williamson, DL
Swartz, RW
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1998, 216 : U324 - U324

← 1 2 3 4 5 →