HPAEC-PAD method for the analysis of alkaline hydrolyzates of Haemophilus influenzae type b capsular polysaccharide

被引：12
|
作者：
de Haan, Alex [1 ]
van der Put, Robert M. F. [1 ]
Beurret, Michel [1 ]
机构：
[1] RIVM, Natl Inst Publ Hlth & Environm, Unit Vaccinol, Ctr Infect Dis Control CIb, NL-3720 BA Bilthoven, Netherlands
来源：
BIOMEDICAL CHROMATOGRAPHY | 2013年 / 27卷 / 09期
关键词：
Haemophilus influenzae type b (Hib); polysaccharide analysis; conjugate vaccine analysis; high performance anion exchange chromatography (HPAEC); pulsed amperometric detection (PAD); alkaline hydrolysis; ANION-EXCHANGE CHROMATOGRAPHY; PULSED-AMPEROMETRIC DETECTION; TOXOID-CONJUGATE VACCINE; HEMOPHILUS-INFLUENZAE; QUANTIFICATION; COMBINATION; SACCHARIDE; STABILITY; ASSAY; PRP;
D O I：
10.1002/bmc.2918
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A gradient method has been devised for the rapid analysis of alkaline hydrolyzates of Haemophilus influenzae type b (Hib) capsular polysaccharide-based vaccines by high-performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD). As compared with published procedures, peak shape and sensitivity were significantly improved with this approach, analysis time was short and there was little interference from impurities. The limits of detection and quantification were established with a purified reference polysaccharide. We propose this method as a practical alternative for the analysis of minute amounts of Hib polysaccharide, which can be lower than with the conventional approaches. Copyright (C) 2013 John Wiley & Sons, Ltd.
引用
收藏
页码：1137 / 1142
页数：6
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