Protective effect of minocycline on dexamethasone induced testicular germ cell apoptosis in mice

被引:0
|
作者
Orazizadeh, M. [1 ]
Hashemitabar, M. [1 ]
Khorsandi, L. [1 ]
机构
[1] Ahwaz Jundi Shapour Univ Med Sci, Dept Anat Sci, Fac Med, Ahvaz, Iran
关键词
Minocycline; Dexametasone; Germ cells; Apoptosis;
D O I
暂无
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Background: Apoptosis involves in testicular germ cell loss in animals and humans, and plays an important role in male fertility. Previous studies have reported neuroprotective and antiapoptotic effects of minocycline. Aim: In this study the protective effect of Minocycline on testicular germ cell apoptosis arising from dexamethasone (Dex) has been evaluated. Dex is a widely used as a glucocorticoid (GC) agent that its apoptotic effect has been shown. Materials and Methods: Experimental groups of 8 male mice received one of the following treatments daily for 7 days: 100 mg/kg Minocycline, 7 mg/kg Dex and 7 mg/kg Dex +100 mg/kg Minocycline. Control group was treated with 0.5 ml saline given orally for a week. Then the mice were sacrificed, and their testes processed for assessment of germ cell apoptosis (TUNEL method), the quality of spermatogenesis (Johnsen score system) and testicular sperm counts. Results: Germ cell apoptosis were significantly increased in Dex treated mice compared with control (P<0.01). Spermatogenesis and the number of sperms head were significantly reduced in Dex treated mice compared with those of the control group (P<0.01). Treatment with Dex and Minocycline resulted in an inhibition effect on germ cell apoptosis and a significant increase in the Johnsen score and the number of head sperm compared with Dex treated mice (P<0.05). Conclusion: The application of Minocycline may serve as a beneficial medication to protect germ cells against apoptosis.
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页码:1 / 5
页数:5
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