Protective effect of minocycline on dexamethasone induced testicular germ cell apoptosis in mice

被引:0
|
作者
Orazizadeh, M. [1 ]
Hashemitabar, M. [1 ]
Khorsandi, L. [1 ]
机构
[1] Ahwaz Jundi Shapour Univ Med Sci, Dept Anat Sci, Fac Med, Ahvaz, Iran
关键词
Minocycline; Dexametasone; Germ cells; Apoptosis;
D O I
暂无
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Background: Apoptosis involves in testicular germ cell loss in animals and humans, and plays an important role in male fertility. Previous studies have reported neuroprotective and antiapoptotic effects of minocycline. Aim: In this study the protective effect of Minocycline on testicular germ cell apoptosis arising from dexamethasone (Dex) has been evaluated. Dex is a widely used as a glucocorticoid (GC) agent that its apoptotic effect has been shown. Materials and Methods: Experimental groups of 8 male mice received one of the following treatments daily for 7 days: 100 mg/kg Minocycline, 7 mg/kg Dex and 7 mg/kg Dex +100 mg/kg Minocycline. Control group was treated with 0.5 ml saline given orally for a week. Then the mice were sacrificed, and their testes processed for assessment of germ cell apoptosis (TUNEL method), the quality of spermatogenesis (Johnsen score system) and testicular sperm counts. Results: Germ cell apoptosis were significantly increased in Dex treated mice compared with control (P<0.01). Spermatogenesis and the number of sperms head were significantly reduced in Dex treated mice compared with those of the control group (P<0.01). Treatment with Dex and Minocycline resulted in an inhibition effect on germ cell apoptosis and a significant increase in the Johnsen score and the number of head sperm compared with Dex treated mice (P<0.05). Conclusion: The application of Minocycline may serve as a beneficial medication to protect germ cells against apoptosis.
引用
收藏
页码:1 / 5
页数:5
相关论文
共 50 条
  • [31] Effect of aging and dietary restriction on rat testicular germ cell apoptosis -: Commentary
    Blanco-Rodríguez, J
    JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 1999, 54 (05): : B205 - B206
  • [32] IGFBP3 ENHANCES GERM CELL APOPTOSIS INDUCED BY TESTICULAR HORMONAL DEPRIVATION
    Lue, Y.
    Mehta, H.
    Liu, Q.
    Cohen, P.
    Jia, Y.
    Hikim, A. Sinha
    Swerdloff, R.
    Wang, C.
    JOURNAL OF INVESTIGATIVE MEDICINE, 2009, 57 (01) : 203 - 203
  • [33] Xanthine oxidase inhibitors suppress testicular germ cell apoptosis induced by experimental cryptorchidism
    Kumagai, A
    Kodama, H
    Kumagai, J
    Fukuda, J
    Kawamura, K
    Tanikawa, H
    Sato, N
    Tanaka, T
    MOLECULAR HUMAN REPRODUCTION, 2002, 8 (02) : 118 - 123
  • [34] IGFBP-3 ENHANCES GERM CELL APOPTOSIS INDUCED BY TESTICULAR HORMONAL DEPRIVATION
    Lue, YanHe
    Mehta, Hemal
    Liu, QingHai
    Cohen, Pinchas
    Jia, Yue
    Hikim, Amiya Sinha
    Swerdloff, Ronald
    Wang, Christina
    JOURNAL OF ANDROLOGY, 2009, : 33 - 33
  • [35] MEHP-induced rat testicular inflammation does not exacerbate germ cell apoptosis
    Voss, Jorine J. L. P.
    Stermer, Angela R.
    Ghaffari, Rashin
    Tiwary, Richa
    Richburg, John H.
    REPRODUCTION, 2018, 156 (01) : 35 - 46
  • [36] Inhibition of testicular germ cell apoptosis and differentiation in mice misexpressing Bcl-2 in spermatogonia
    Furuchi, T
    Masuko, K
    Nishimune, Y
    Obinata, M
    Matsui, Y
    DEVELOPMENT, 1996, 122 (06): : 1703 - 1709
  • [37] Protective Effects of Tribulus terrestris Hydroalcoholic Extract Against Cisplatin-Induced Germ Cell Apoptosis in Male Mice
    Zahra, Keshtmand
    INTERNATIONAL JOURNAL OF MORPHOLOGY, 2018, 36 (01): : 140 - 144
  • [38] Effects of 60 Hz 14 μT Magnetic Field on the Apoptosis of Testicular Germ Cell in Mice
    Kim, Yoon-Won
    Kim, Hee-Sung
    Lee, Jin-Sang
    Kim, Young-Jin
    Lee, Sang-Kon
    Seo, Jae-Nam
    Jung, Kyeong-Cheon
    Kim, Nam
    Gimm, Youn-Myoung
    BIOELECTROMAGNETICS, 2009, 30 (01) : 66 - 72
  • [39] Fas- or FasL-deficient mice display an increased sensitivity to nitrobenzene-induced testicular germ cell apoptosis
    Richburg, JH
    Nañez, A
    TOXICOLOGY LETTERS, 2003, 139 (01) : 1 - 10
  • [40] Overexpression of endothelial nitric oxide synthase (eNOS) in transgenic mice accelerates testicular germ cell apoptosis induced by experimental cryptorchidism
    Ishikawa, T
    Fujisawa, M
    Kondo, Y
    Goda, K
    Kamidono, S
    JOURNAL OF UROLOGY, 2004, 171 (04): : 364 - 364