Co-expression of p16/ki-67 in Associated with Human Papilloma Virus Type 16

The current trend in screening against cervical cancer is to improve the sensitivity of screening method and p16/Ki-67 dual-stained cytology, represents a promising approach. We performed a study to investigate the correlation between the p16/Ki-67 co-expression and HPV type on a group of patients with ASCUS and LSIL on Pap smear. On all patients, dual stain test and HPV genotyping were performed. In the group of patients with positive dual stain p16/Ki-67 test, the number of patients with HPV type 16 was significantly higher than the number of patients with any other type of HPV. Also, all patients with CIN3 or above (cervical cancer) were positive for p16/Ki-67. Our study shows the potential use of those two tests to identify patients with high risk for severe dysplasia.