Identification of domestic rice cultivars by RAPD analysis using one grain of milled rice as a sample

Simple and rapid extraction methods for DNA extraction from single milled rice grains were investigated for cultivar identification of domestic rices by RAPD analysis. Single grain of milled rice was treated with ISOPLANT kit (Nippon Gene) or FastDNA kit H (BIO 101), and extracted DNA solutions were used for PCR amplification. These extraction methods were applied for RAPD analysis to identify 10 dominant cultivars in Japan. RAPD data using DNA extracts from one grain of milled rice were useful for the cultivar identification of rice when the suitable primers were used in RAPD analysis. It became possible that 10 dominant rice cultivars in Japan were identified by RAPD analysis from one grain of milled rice using 5 primers and 6 discrimination bands.