Label-free detection of DNA hybridization based on EIS investigation of conducting properties of functionalized polythiophene matrix

被引：39
|
作者：
Gautier, C
Cougnon, C
Pilard, JF
Casse, N
机构：
[1] Univ Mainz, CNRS, UC03M, UMR 6011,LCOM Chim Polymeres, F-72085 Le Mans 9, France
[2] Univ Maine, Lab Biol & Genet Evolut, F-72085 Le Mans 9, France
来源：
JOURNAL OF ELECTROANALYTICAL CHEMISTRY | 2006年 / 587卷 / 02期
关键词：
DNA sensor; free-label detection; DNA-modified polythiophene; electrochemical impedance spectroscopy;
D O I：
10.1016/j.jelechem.2005.11.021
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A conducting polythiophene sensor for direct label-free DNA detection is investigated by electrochemical impedance spectroscopy (EIS) and by quartz crystal microbalance. The end-modified DNA probe is covalently immobilized by using a new anchorage function. The hybridization detection is studied by EIS experiments. An equivalent circuit is suggested and the variations of individual values of parameters deduced from impedance fits are connected to the change of conformation during the hybridization process. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码：276 / 283
页数：8
相关论文
共 50 条
  • [21] Label-free detection of DNA hybridization using transistors based on CVD grown graphene
    Lin, C.-T. (chengte.lin@gmail.com), 1600, Elsevier Ltd (41):
  • [22] Label-free detection of DNA hybridization using transistors based on CVD grown graphene
    Chen, Tzu-Yin
    Phan Thi Kim Loan
    Hsu, Chang-Lung
    Lee, Yi-Hsien
    Wang, Jacob Tse-Wei
    Wei, Kung-Hwa
    Lin, Cheng-Te
    Li, Lain-Jong
    BIOSENSORS & BIOELECTRONICS, 2013, 41 : 103 - 109
  • [23] Label-free detection of DNA hybridization using nanopillar arrays based optical biosensor
    Chen, Jem-Kun
    Zhou, Gang-Yan
    Chang, Chi-Jung
    Cheng, Chih-Chia
    SENSORS AND ACTUATORS B-CHEMICAL, 2014, 194 : 10 - 18
  • [24] Pencil-based renewable biosensor for label-free electrochemical detection of DNA hybridization
    Wang, J
    Kawde, AN
    ANALYTICA CHIMICA ACTA, 2001, 431 (02) : 219 - 224
  • [25] Label-free slot-waveguide biosensor for the detection of DNA hybridization
    Dar, Tuffail
    Homola, Jiri
    Rahman, B. M. Azizur
    Rajarajan, Muttukrishnan
    APPLIED OPTICS, 2012, 51 (34) : 8195 - 8202
  • [26] Photonic crystals on copolymer film for label-free detection of DNA hybridization
    Su, Han
    Cheng, Xin R.
    Endo, Tatsuro
    Kerman, Kagan
    BIOSENSORS & BIOELECTRONICS, 2018, 103 : 158 - 162
  • [27] Nanoporous niobium oxide for label-free detection of DNA hybridization events
    Choi, Jinsub
    Lim, Jae Hoon
    Rho, Sangchul
    Jahng, Deokjin
    Lee, Jaeyoung
    Kim, Kyung Ja
    TALANTA, 2008, 74 (04) : 1056 - 1059
  • [28] Biosensor for label-free DNA quantification based on functionalized LPGs
    Goncalves, Helena M. R.
    Moreira, Luis
    Pereira, Leonor
    Jorge, Pedro
    Gouveia, Carlos
    Martins-Lopes, Paula
    Fernandes, Jose R. A.
    BIOSENSORS & BIOELECTRONICS, 2016, 84 : 30 - 36
  • [29] Synthesis of a functionalized polythiophene as an active substrate for a label-free electrochemical genosensor
    Peng, Hui
    Zhang, Lijuan
    Spires, John
    Soeller, Christian
    Travas-Sejdic, Jadranka
    POLYMER, 2007, 48 (12) : 3413 - 3419
  • [30] Fabrication of DNA/graphene/polyaniline nanocomplex for label-free voltammetric detection of DNA hybridization
    Du, Meng
    Yang, Tao
    Li, Xiao
    Jiao, Kui
    TALANTA, 2012, 88 : 439 - 444
12345