Study of van Gieson's picrofuchsin staining on second-harmonic generation in type I collagen

被引:1
|
作者
Liu, Hanping [1 ]
Zhuang, Zhengfei [1 ]
Zhao, Lingling [2 ]
Qu, Junle [2 ]
Deng, Xiaoyuan [1 ]
机构
[1] S China Normal Univ, Sch Informat & Optoelect Sci & Engn, Guangzhou 510700, Guangdong, Peoples R China
[2] Shenzhen Univ, Minist Educ & Guangdong Prov, Key Lab Optoelect Devices & Syst, Inst Optoelect, Shenzhen 518060, Peoples R China
基金
中国国家自然科学基金;
关键词
D O I
10.3788/COL20080612.0882
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Second-harmonic generation (SHG) microscopy is a recently developed nonlinear optical imaging modality for imaging tissue structures with submicron resolution and is a potent tool for visualizing pathological effects of diseases. In this letter, we present our investigation on the influence of van Gieson's (VG) alcoholic picrofuchsin staining on SHC in type I collagen (from tendon-rich C57BL/6). Multi-channel imaging and spectra analysis show that the strong SHC signal produced in fresh collagen type I fiber has been greatly suppressed after VC staining, which indicates that staining may induce the structural or characteristic changes of SHG-dependent crystal formed by collagen constituents, such as glycine, proline, and hydroxyproline.
引用
收藏
页码:882 / 885
页数:4
相关论文
共 50 条
  • [31] Study on stability and chaos of optical second-harmonic generation
    Zhang, XH
    Shen, K
    ELECTRO-OPTIC AND SECOND HARMONIC GENERATION MATERIALS, DEVICES, AND APPLICATIONS II, 1998, 3556 : 233 - 238
  • [32] STUDY OF DISORDERED CRYSTALS BY OPTICAL SECOND-HARMONIC GENERATION
    VOGT, H
    PHYSICA STATUS SOLIDI B-BASIC RESEARCH, 1973, 58 (02): : 705 - 714
  • [33] Second harmonic generation signal from type I collagen fibers grown in vitro
    Grethel Fuentes-Corona, Cindy
    Licea-Rodriguez, Jacob
    Younger, Rebecca
    Rangel-Rojo, Raul
    Potma, Eric O.
    Rocha-Mendoza, Israel
    BIOMEDICAL OPTICS EXPRESS, 2019, 10 (12) : 6449 - 6461
  • [34] Polarization second harmonic generation by image correlation spectroscopy on collagen type I hydrogels
    Paesen, Rik
    Sanen, Kathleen
    Smisdom, Nick
    Michiels, Luc
    Ameloot, Marcel
    ACTA BIOMATERIALIA, 2014, 10 (05) : 2036 - 2042
  • [35] Second harmonic generation in two different arrangement of type I collagen on nonlinear microscopy
    Chen, Ying
    Jiang, Guobao
    Zhou, Yuan
    Xia, Xu
    RESULTS IN PHYSICS, 2018, 10 : 391 - 394
  • [36] Second-harmonic generation imaging of collagen fibers in myocardium for atrial fibrillation diagnosis
    Tsai, Ming-Rung
    Chiu, Yu-Wei
    Lo, Men Tzung
    Sun, Chi-Kuang
    JOURNAL OF BIOMEDICAL OPTICS, 2010, 15 (02)
  • [37] Quantifying collagen structure in breast biopsies using second-harmonic generation imaging
    Ambekar, Raghu
    Lau, Tung-Yuen
    Walsh, Michael
    Bhargava, Rohit
    Toussaint, Kimani C., Jr.
    BIOMEDICAL OPTICS EXPRESS, 2012, 3 (09): : 2021 - 2035
  • [38] Multiscale anisotropy analysis of second-harmonic generation collagen imaging of mouse skin
    Tilbury, Karissa
    Han, XiangHua
    Brooks, Peter
    Khalil, Andre
    JOURNAL OF BIOMEDICAL OPTICS, 2021, 26 (06)
  • [39] van der Waals NbOCl2 Nanodisks for Enhanced Second-Harmonic Generation
    Chu, Lingrui
    Gao, Siying
    Li, Ziqi
    Zhu, Han
    Liu, Weijie
    Jia, Yuechen
    Chen, Feng
    NANO LETTERS, 2024, 24 (48) : 15457 - 15463
  • [40] Miller's rule and the static limit for second-harmonic generation
    Scandolo, S.
    Bassani, F.
    Physical Review B: Condensed Matter, 1995, 516