Study of van Gieson's picrofuchsin staining on second-harmonic generation in type I collagen

被引:1
|
作者
Liu, Hanping [1 ]
Zhuang, Zhengfei [1 ]
Zhao, Lingling [2 ]
Qu, Junle [2 ]
Deng, Xiaoyuan [1 ]
机构
[1] S China Normal Univ, Sch Informat & Optoelect Sci & Engn, Guangzhou 510700, Guangdong, Peoples R China
[2] Shenzhen Univ, Minist Educ & Guangdong Prov, Key Lab Optoelect Devices & Syst, Inst Optoelect, Shenzhen 518060, Peoples R China
基金
中国国家自然科学基金;
关键词
D O I
10.3788/COL20080612.0882
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Second-harmonic generation (SHG) microscopy is a recently developed nonlinear optical imaging modality for imaging tissue structures with submicron resolution and is a potent tool for visualizing pathological effects of diseases. In this letter, we present our investigation on the influence of van Gieson's (VG) alcoholic picrofuchsin staining on SHC in type I collagen (from tendon-rich C57BL/6). Multi-channel imaging and spectra analysis show that the strong SHC signal produced in fresh collagen type I fiber has been greatly suppressed after VC staining, which indicates that staining may induce the structural or characteristic changes of SHG-dependent crystal formed by collagen constituents, such as glycine, proline, and hydroxyproline.
引用
收藏
页码:882 / 885
页数:4
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