Characterization of virus-like particles by atomic force microscopy in ambient conditions

被引：5
|
作者：
Oropesa, Reinier [1 ]
Ramos, Jorge R. [1 ]
Falcon, Viviana [2 ]
Felipe, Ariel [3 ]
机构：
[1] Ctr Adv Studies Cuba, Havana, Cuba
[2] Ctr Genet Engn & Biotechnol, Havana, Cuba
[3] Council State, Sci Advisors Off, Havana, Cuba
来源：
ADVANCES IN NATURAL SCIENCES-NANOSCIENCE AND NANOTECHNOLOGY | 2013年 / 4卷 / 02期
关键词：
hepatitis B; AFM; rHBsAg; virus-like particles;
D O I：
10.1088/2043-6262/4/2/025007
中图分类号：
TB3 [工程材料学];
学科分类号：
0805 ; 080502 ;
摘要：
Recombinant virus-like particles (VLPs) are attractive candidates for vaccine design since they resemble native viroids in size and morphology, but they are non-infectious due to the absence of a viral genome. The visualization of surface morphologies and structures can be used to deepen the understanding of physical, chemical, and biological phenomena. Atomic force microscopy (AFM) is a useful tool for the visualization of soft biological samples in a nanoscale resolution. In this work we have investigated the morphology of recombinant surface antigens of hepatitis B (rHBsAg) VLPs from Cuban vaccine against hepatitis B. The rHBsAg VLPs sizes estimated by AFM between 15 and 30 nm are similar to those reported on previous transmission electron microscopy (TEM) studies.
引用
收藏
页数：4
相关论文
共 50 条
  • [41] Investigation of erythrocyte membrane and virus particles interaction by atomic force microscopy
    Zajtsev, B.N.
    Durymanov, A.G.
    Bakulina, L.F.
    Generalov, V.M.
    Poverkhnost Rentgenovskie Sinkhronnye i Nejtronnye Issledovaniya, 2003, (07): : 34 - 39
  • [42] Atomic force microscopy at ambient and liquid conditions with stiff sensors and small amplitudes
    Wutscher, Elisabeth
    Giessibl, Franz J.
    REVIEW OF SCIENTIFIC INSTRUMENTS, 2011, 82 (09):
  • [43] VIRUS AND VIRUS-LIKE PARTICLES IN HUMAN STOOLS
    FALCIERI, E
    MUSIANI, M
    PICCARI, GG
    ULTRAMICROSCOPY, 1980, 5 (03) : 426 - 426
  • [44] Imaging Water Thin Films in Ambient Conditions Using Atomic Force Microscopy
    Santos, Sergio
    Verdaguer, Albert
    MATERIALS, 2016, 9 (03):
  • [45] SMALL, VIRUS-LIKE PARTICLES DETECTED IN BOVINE SERA BY ELECTRON-MICROSCOPY
    BENZ, EW
    MOSES, HL
    JOURNAL OF THE NATIONAL CANCER INSTITUTE, 1974, 52 (06) : 1931 - 1934
  • [46] Electron Microscopy Reveals Variance in the Assembly of Mutant Qβ Virus-Like Particles
    Campbell, Melody G.
    Fiedler, Jason D.
    Higginson, Cody
    Kislukhin, Alexander
    Castillejos, Alexandra
    Manzenreider, Florian
    Potter, Clinton S.
    Carragher, Bridget
    Finn, M. G.
    FASEB JOURNAL, 2012, 26
  • [47] THE CYTOLOGY OF INCLUSION BODY PAPILLOMAS YIELDING VIRUS-LIKE PARTICLES BY ELECTRON MICROSCOPY
    BUNTING, H
    STRAUSS, MJ
    MELNICK, JL
    AMERICAN JOURNAL OF PATHOLOGY, 1951, 27 (04): : 735 - 736
  • [48] IMMUNE ELECTRON-MICROSCOPY OF VIRUS-LIKE PARTICLES OF AGARICUS-BISPORUS
    DELVECCHIO, VG
    DIXON, C
    LEMKE, PA
    EXPERIMENTAL MYCOLOGY, 1978, 2 (02): : 138 - 144
  • [49] Expression and characterization of hepatitis E virus-like particles and non-virus-like particles from insect cells
    Qi, Ying
    Fan, Jinping
    Huang, Weijin
    Zhao, Chenyan
    Wang, Youchun
    Kong, Franklin T.
    Kong, Wei
    Jiang, Chunlai
    BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY, 2016, 63 (03) : 362 - 370
  • [50] Virus-like Particles Armored by an Endoskeleton
    Wu, Zhuohong
    Bayon, Jorge L.
    Kouznetsova, Tatiana B.
    Ouchi, Tetsu
    Barkovich, Krister J.
    Hsu, Sean K.
    Craig, Stephen L.
    Steinmetz, Nicole F.
    NANO LETTERS, 2024, 24 (10) : 2989 - 2997
12345