Characterization of virus-like particles by atomic force microscopy in ambient conditions

被引：5

作者：

Oropesa, Reinier ^{[1
]}

Ramos, Jorge R. ^{[1
]}

Falcon, Viviana ^{[2
]}

Felipe, Ariel ^{[3
]}

机构：

[1] Ctr Adv Studies Cuba, Havana, Cuba

[2] Ctr Genet Engn & Biotechnol, Havana, Cuba

[3] Council State, Sci Advisors Off, Havana, Cuba

来源：

ADVANCES IN NATURAL SCIENCES-NANOSCIENCE AND NANOTECHNOLOGY | 2013年 / 4卷 / 02期

关键词：

hepatitis B; AFM; rHBsAg; virus-like particles;

D O I：

10.1088/2043-6262/4/2/025007

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Recombinant virus-like particles (VLPs) are attractive candidates for vaccine design since they resemble native viroids in size and morphology, but they are non-infectious due to the absence of a viral genome. The visualization of surface morphologies and structures can be used to deepen the understanding of physical, chemical, and biological phenomena. Atomic force microscopy (AFM) is a useful tool for the visualization of soft biological samples in a nanoscale resolution. In this work we have investigated the morphology of recombinant surface antigens of hepatitis B (rHBsAg) VLPs from Cuban vaccine against hepatitis B. The rHBsAg VLPs sizes estimated by AFM between 15 and 30 nm are similar to those reported on previous transmission electron microscopy (TEM) studies.

引用

页数：4

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