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Development of 10 highly-polymorphic microsatellite markers in the vulnerable Galapagos land iguanas (genus Conolophus)
被引:2
|作者:
Rosa, Sabrina F. P.
[1
]
Monteyne, Daniel
[1
]
Milinkovitch, Michel C.
[1
,2
]
机构:
[1] Univ Libre Bruxelles, Inst Mol Biol & Med, Lab Evolutionary Genet, B-6041 Gosselies, Belgium
[2] Lab Nat & Artificial Evolut, CH-1211 Geneva 4, Switzerland
关键词:
Conolophus;
conservation genetics;
Galapagos land iguanas;
microsatellite;
population genetics;
REPATRIATION;
DNA;
D O I:
10.1111/j.1755-0998.2008.02457.x
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The two species of Galapagos land iguanas (Conolophus subcristatus and C. pallidus) are listed as 'vulnerable' species by the International Union for the Conservation of Nature (IUCN Red List; www.iucnredlist.org). Here, we report on the isolation and characterization of 10 microsatellite markers using 562 individuals sampled on all Galapagos islands where Conolophus species occur today. We show that these 10 loci are highly polymorphic and display diagnostic alleles for five out of the six island populations. These markers will be useful for Conolophus population genetic analyses as well as for guiding ongoing captive breeding programmes.
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页码:376 / 379
页数:4
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