Development of 10 highly-polymorphic microsatellite markers in the vulnerable Galapagos land iguanas (genus Conolophus)

被引:2
|
作者
Rosa, Sabrina F. P. [1 ]
Monteyne, Daniel [1 ]
Milinkovitch, Michel C. [1 ,2 ]
机构
[1] Univ Libre Bruxelles, Inst Mol Biol & Med, Lab Evolutionary Genet, B-6041 Gosselies, Belgium
[2] Lab Nat & Artificial Evolut, CH-1211 Geneva 4, Switzerland
关键词
Conolophus; conservation genetics; Galapagos land iguanas; microsatellite; population genetics; REPATRIATION; DNA;
D O I
10.1111/j.1755-0998.2008.02457.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The two species of Galapagos land iguanas (Conolophus subcristatus and C. pallidus) are listed as 'vulnerable' species by the International Union for the Conservation of Nature (IUCN Red List; www.iucnredlist.org). Here, we report on the isolation and characterization of 10 microsatellite markers using 562 individuals sampled on all Galapagos islands where Conolophus species occur today. We show that these 10 loci are highly polymorphic and display diagnostic alleles for five out of the six island populations. These markers will be useful for Conolophus population genetic analyses as well as for guiding ongoing captive breeding programmes.
引用
收藏
页码:376 / 379
页数:4
相关论文
共 36 条
  • [21] Development and Characterization of 15 Polymorphic Microsatellite Markers for a Highly Adaptive and Wide-range Frog (Microhyla fissipes)
    Zhang, Jing
    Wang, Jie
    Jiang, Jianping
    ASIAN HERPETOLOGICAL RESEARCH, 2013, 4 (03) : 202 - 206
  • [22] Isolation and characterization of 10 highly polymorphic di- and trinucleotide microsatellite markers in the mayfly Ameletus inopinatus (Ephemeroptera: Siphlonuridae)
    Theissinger, Kathrin
    Feldheim, Kevin A.
    Taubmann, Julia
    Seitz, Alfred
    Pauls, Steffen U.
    MOLECULAR ECOLOGY RESOURCES, 2008, 8 (06) : 1285 - 1287
  • [23] Development and Characterization of 15 Polymorphic Microsatellite Markers for a Highly Adaptive and Wide-range Frog (Microhyla f issipes)
    Jing ZHANG
    Jie WANG
    Jianping JIANG
    Asian Herpetological Research, 2013, (03) : 202 - 206
  • [24] Development and Characterization of 15 Polymorphic Microsatellite Markers for a Highly Adaptive and Wide-range Frog (Microhyla f issipes)
    Jing ZHANG
    Jie WANG
    Jianping JIANG
    AsianHerpetologicalResearch, 2013, 4 (03) : 202 - 206
  • [25] Development of 10 polymorphic microsatellite markers from herbicide-bleached tissues of the brooding pocilloporid coral Seriatopora hystrix
    Underwood, JN
    Souter, PB
    Ballment, ER
    Lutz, AH
    van Oppen, MJH
    MOLECULAR ECOLOGY NOTES, 2006, 6 (01): : 176 - 178
  • [26] Development of highly conserved primers for 12 new polymorphic microsatellite loci for the genus Rorippa Scop. (Brassicaceae), yellow-cress
    Stift, Marc
    Kuperus, Peter
    Van Tienderen, Peter H.
    MOLECULAR ECOLOGY NOTES, 2006, 6 (04): : 1129 - 1131
  • [27] Development of ten highly polymorphic microsatellite markers for Sunbirds (Aves: Nectariniidae) with broad cross-amplification utility in at least eight species
    Bowie, Rauri C. K.
    Sellas, Anna B.
    Feldheim, Kevin A.
    CONSERVATION GENETICS, 2010, 11 (03) : 1159 - 1162
  • [28] Development of ten highly polymorphic microsatellite markers for Sunbirds (Aves: Nectariniidae) with broad cross-amplification utility in at least eight species
    Rauri C. K. Bowie
    Anna B. Sellas
    Kevin A. Feldheim
    Conservation Genetics, 2010, 11 : 1159 - 1162
  • [29] Development of the first polymorphic microsatellite markers for the Roman snail Helix pomatia L., 1758 (Helicidae) and cross-species amplification within the genus Helix
    Krapal, A. M.
    Popa, O. P.
    Iorgu, E. I.
    Cojocaru, L. B.
    Popa, A. F.
    Popa, L. O.
    GENETICS AND MOLECULAR RESEARCH, 2016, 15 (03)
  • [30] The development of 10 novel polymorphic microsatellite markers through next generation sequencing and a preliminary population genetic analysis for the endangered Glenelg spiny crayfish, Euastacus bispinosus
    Adam D. Miller
    Anthony Van Rooyen
    Oisín F. Sweeney
    Nick S. Whiterod
    Andrew R. Weeks
    Molecular Biology Reports, 2013, 40 : 4415 - 4419