An acid phosphatase from Manihot glaziovii as an alternative to alkaline phosphatase for molecular cloning experiments

被引：1
|
作者：
Tham, SC
Lim, SH
Yeoh, HH [1 ]
机构：
[1] Natl Univ Singapore, Fac Sci, Dept Biol Sci, Singapore 119260, Singapore
[2] Malaysia Univ Sci & Technol, Biotechnol Program, Petaling Jaya, Malaysia
来源：
BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 23-24期
关键词：
acid phosphatase; dephosphorylation; enzyme kinetics; Manihot glaziovii;
D O I：
10.1007/s10529-005-3894-z
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An acid phosphatase, free of deoxyribonuclease activity, was isolated from Manihot glaziovii leaves. It had a Mr of 78 kDa and was optimally active at pH 4.3 and 52 degrees C. It was inactivated at 65 degrees C over 15 min. It had a broad substrate specificity with strongest activity towards p-nitrophenyl phosphate. The enzyme dephosphorylated linearized pUC18 DNA and preventing self-ligation under the same conditions used for calf intestine alkaline phosphatase.
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页码：1865 / 1868
页数：4
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