An acid phosphatase from Manihot glaziovii as an alternative to alkaline phosphatase for molecular cloning experiments

被引：1
|
作者：
Tham, SC
Lim, SH
Yeoh, HH [1 ]
机构：
[1] Natl Univ Singapore, Fac Sci, Dept Biol Sci, Singapore 119260, Singapore
[2] Malaysia Univ Sci & Technol, Biotechnol Program, Petaling Jaya, Malaysia
来源：
BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 23-24期
关键词：
acid phosphatase; dephosphorylation; enzyme kinetics; Manihot glaziovii;
D O I：
10.1007/s10529-005-3894-z
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An acid phosphatase, free of deoxyribonuclease activity, was isolated from Manihot glaziovii leaves. It had a Mr of 78 kDa and was optimally active at pH 4.3 and 52 degrees C. It was inactivated at 65 degrees C over 15 min. It had a broad substrate specificity with strongest activity towards p-nitrophenyl phosphate. The enzyme dephosphorylated linearized pUC18 DNA and preventing self-ligation under the same conditions used for calf intestine alkaline phosphatase.
引用
收藏
页码：1865 / 1868
页数：4
相关论文
共 50 条
  • [31] A Molecular Description of Acid Phosphatase
    Anand, Asha
    Srivastava, Pramod Kumar
    APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2012, 167 (08) : 2174 - 2197
  • [32] Acid and alkaline phosphatase and nucleo-phosphatase in the erythrocytes of some lower vertebrates
    Rapoport, S
    Leva, E
    Guest, GM
    JOURNAL OF CELLULAR AND COMPARATIVE PHYSIOLOGY, 1942, 19 (01): : 103 - 108
  • [33] In vitro experiments and infrared spectroscopy analysis of acid and alkaline phosphatase inhibition by vanadium complexes
    Parente, Juliana E.
    Naso, Luciana G.
    Jori, Khalil
    Franca, Carlos A.
    da Costa Ferreira, Ana Maria
    Williams, Patricia A. M.
    Ferrer, Evelina G.
    NEW JOURNAL OF CHEMISTRY, 2019, 43 (45) : 17603 - 17619
  • [34] CLONING OF HUMAN LYSOSOMAL ACID-PHOSPHATASE
    POHLMANN, R
    KRENTLER, C
    SCHMIDT, B
    SCHRODER, W
    LORKOWSKI, G
    CULLY, J
    MERSMANN, G
    GRZESCHIK, KH
    HASILIK, A
    VONFIGURA, K
    BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1987, 368 (09): : 1094 - 1094
  • [35] THE DIFFUSION COEFFICIENT AND MOLECULAR WEIGHT OF ALKALINE PHOSPHATASE
    MATHIES, JC
    GOODMAN, ED
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1953, 75 (23) : 6061 - 6062
  • [36] ALKALINE-PHOSPHATASE INHIBITS CLONING IN STREPTOCOCCUS-PNEUMONIAE
    POZZI, G
    GUILD, WR
    FEMS MICROBIOLOGY LETTERS, 1987, 41 (03) : 309 - 311
  • [37] CLONING AND SEQUENCING OF HUMAN INTESTINAL ALKALINE-PHOSPHATASE CDNA
    BERGER, J
    GARATTINI, E
    HUA, JC
    UDENFRIEND, S
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (03) : 695 - 698
  • [38] CLONING AND SEQUENCING OF BOVINE KIDNEY ALKALINE-PHOSPHATASE CDNA
    GARATTINI, E
    HUA, JC
    UDENFRIEND, S
    GENE, 1987, 59 (01) : 41 - 46
  • [39] EXPERIMENTS ON ENZYMIC DEPHOSPHORYLATION OF NADP ANALOGUES BY ALKALINE PHOSPHATASE FROM CALF GUT
    CHRIST, W
    SCHMIDT, D
    COPER, H
    HOPPE-SEYLERS ZEITSCHRIFT FUR PHYSIOLOGISCHE CHEMIE, 1970, 351 (07): : 803 - +
  • [40] Characterization of alkaline phosphatase genes expressed in seminoma by cDNA cloning
    Shigenari, A
    Ando, A
    Baba, T
    Yamamoto, T
    Katsuoka, Y
    Inoko, H
    CANCER RESEARCH, 1998, 58 (22) : 5079 - 5082
12345