An acid phosphatase from Manihot glaziovii as an alternative to alkaline phosphatase for molecular cloning experiments

被引：1
|
作者：
Tham, SC
Lim, SH
Yeoh, HH [1 ]
机构：
[1] Natl Univ Singapore, Fac Sci, Dept Biol Sci, Singapore 119260, Singapore
[2] Malaysia Univ Sci & Technol, Biotechnol Program, Petaling Jaya, Malaysia
来源：
BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 23-24期
关键词：
acid phosphatase; dephosphorylation; enzyme kinetics; Manihot glaziovii;
D O I：
10.1007/s10529-005-3894-z
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
An acid phosphatase, free of deoxyribonuclease activity, was isolated from Manihot glaziovii leaves. It had a Mr of 78 kDa and was optimally active at pH 4.3 and 52 degrees C. It was inactivated at 65 degrees C over 15 min. It had a broad substrate specificity with strongest activity towards p-nitrophenyl phosphate. The enzyme dephosphorylated linearized pUC18 DNA and preventing self-ligation under the same conditions used for calf intestine alkaline phosphatase.
引用
收藏
页码：1865 / 1868
页数：4
相关论文
共 50 条
  • [1] An Acid Phosphatase from Manihot glaziovii as an Alternative to Alkaline Phosphatase for Molecular Cloning Experiments
    S.C. Tham
    S.H. Lim
    H.H. Yeoh
    Biotechnology Letters, 2005, 27 : 1865 - 1868
  • [2] Immobilized alkaline phosphatase for molecular cloning
    Zubriene, A
    Budriene, S
    Lubiene, J
    Dienys, G
    BIOCATALYSIS AND BIOTRANSFORMATION, 2002, 20 (06) : 423 - 427
  • [3] Molecular cloning, sequencing and alternative splicing of the acid phosphatase (Acph) gene from Drosophila virilis
    Kitagawa, HT
    DNA SEQUENCE, 2003, 14 (02): : 135 - 139
  • [4] ALKALINE AND ACID PHOSPHATASE
    CARR, JJ
    STANDARD METHODS OF CLINICAL CHEMISTRY, 1953, 1 : 75 - 83
  • [5] Cloning of soluble alkaline phosphatase cDNA and molecular basis of the polymorphic nature in alkaline phosphatase isozymes of Bombyx mori midgut
    Itoh, M
    Kanamori, Y
    Takao, M
    Eguchi, M
    INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1999, 29 (02) : 121 - 129
  • [6] Alkaline phosphatase fusion proteins for molecular characterization and cloning of receptors and their ligands
    Flanagan, JG
    Cheng, HJ
    APPLICATIONS OF CHIMERIC GENES AND HYBRID PROTEINS PT B: CELL BIOLOGY AND PHYSIOLOGY, 2000, 327 : 198 - 210
  • [7] Substrate analysis and molecular cloning of the extracellular alkaline phosphatase of Streptomyces griseus
    Moura, RS
    Martín, JF
    Martín, A
    Liras, P
    MICROBIOLOGY-SGM, 2001, 147 : 1525 - 1533
  • [8] MOLECULAR-CLONING OF HUMAN PLACENTAL ALKALINE-PHOSPHATASE CDNA
    MILLAN, JL
    FISHMAN, WH
    TUMOUR BIOLOGY, 1985, 6 (04): : 380 - 380
  • [9] Cloning and expression of alkaline phosphatase gene from Schizosaccharomyces pombe
    Kang, SW
    Cho, YW
    Park, EH
    Ahn, KS
    Lim, CJ
    JOURNAL OF BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2001, 34 (03): : 262 - 267
  • [10] MOLECULAR-CLONING OF THE MOUSE LYSOSOMAL ACID-PHOSPHATASE
    GEIER, C
    VONFIGURA, K
    POHLMANN, R
    BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1991, 372 (04): : 301 - 304
12345