Molecular cloning, expression and characterization of the Canis familiaris interleukin-4

被引：13
|
作者：
Wondimu, A
Veit, M
Kohn, B
Kaul, S
Hoffmann, A
Brunnberg, L
Schmidt, MFG
机构：
[1] Free Univ Berlin, Inst Immunol & Mol Biol, D-10115 Berlin, Germany
[2] Free Univ Berlin, Clin Small Anim, D-10115 Berlin, Germany
[3] Paul Ehrlich Inst, D-63225 Langen, Germany
来源：
CYTOKINE | 2001年 / 16卷 / 03期
关键词：
interleukin; 4; canine cytokines; recombinant cytokines; expression vectors;
D O I：
10.1006/cyto.2001.0953
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Interleukin 4 (IL-4) is an important regulatory cytokine produced by activated T lymphocytes and mast cells, and regulates the growth and differentiation of cells such as B and T lymphocytes. The rapid amplification of cDNA ends (RACE) was used to clone the canine IL-4 gene. It was expressed in mammalian cells and Escherichia coli. Monoclonal antibodies were raised to rcIL-4 for use in an enzyme-linked immunosorbent assay (ELISA). This will facilitate studies on the role of cIL-4 in inflammatory diseases, particularly rheumatoid arthritis. (C) 2001 Academic Press.
引用
收藏
页码：88 / 92
页数：5
相关论文
共 50 条
  • [41] THE INTERLEUKIN-2 AND INTERLEUKIN-4 RECEPTORS STUDIED BY MOLECULAR MODELING
    BAMBOROUGH, P
    HEDGECOCK, CJR
    RICHARDS, WG
    STRUCTURE, 1994, 2 (09) : 839 - 851
  • [42] Expression of interleukin-4 and interleukin-13 and their receptors in colorectal cancer
    Andrea Formentini
    Philipp Braun
    Harald Fricke
    Karl-Heinrich Link
    Doris Henne-Bruns
    Marko Kornmann
    International Journal of Colorectal Disease, 2012, 27 : 1369 - 1376
  • [43] Expression of interleukin-4 and interleukin-13 and their receptors in colorectal cancer
    Formentini, Andrea
    Braun, Philipp
    Fricke, Harald
    Link, Karl-Heinrich
    Henne-Bruns, Doris
    Kornmann, Marko
    INTERNATIONAL JOURNAL OF COLORECTAL DISEASE, 2012, 27 (10) : 1369 - 1376
  • [44] The molecular basis of interleukin-4 induced macrophage fusion
    Helming, L.
    Winter, J.
    Maroof, N.
    Tomasello, E.
    Vivier, E.
    Gordon, S.
    IMMUNOLOGY, 2008, 125 : 4 - 4
  • [45] EFFECTS OF INTERLEUKIN-4 ON PROTEIN EXPRESSION PROFILES IN ADIPOCYTES
    Chang, Y. H.
    Shiau, M. Y.
    DIABETES TECHNOLOGY & THERAPEUTICS, 2014, 16 : A128 - A129
  • [46] Downregulation of decorin expression in dermal fibroblasts by interleukin-4
    K. Kuroda
    Hiroshi Shinkai
    Archives of Dermatological Research, 1997, 289 : 476 - 480
  • [47] INTERLEUKIN-4 GENE-EXPRESSION AND IGE RESPONSIVENESS
    OKUDAIRA, H
    MORI, A
    AKIYOSHI, K
    YAMAMOTO, K
    SUKO, M
    WATANABE, N
    ITO, M
    TAKAHASHI, K
    JUJI, T
    INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1991, 94 (1-4): : 184 - 186
  • [48] Genes that regulate interleukin-4 expression in T cells
    Szabo, SJ
    Glimcher, LH
    Ho, IC
    CURRENT OPINION IN IMMUNOLOGY, 1997, 9 (06) : 776 - 781
  • [49] Toxocara canis:: Molecular cloning, characterization, expression and comparison of the kinetics of cDNA-derived arginine kinase
    Wickramasinghe, Susiji
    Uda, Kouji
    Nagataki, Mitsuru
    Yatawara, Lalani
    Rajapakse, R. P. V. J.
    Watanabe, Yoshiya
    Suzuki, Tomohiko
    Agatsuma, Takeshi
    EXPERIMENTAL PARASITOLOGY, 2007, 117 (02) : 124 - 132
  • [50] GROWTH-INHIBITION OF HUMAN COLORECTAL-CARCINOMA CELLS BY INTERLEUKIN-4 AND EXPRESSION OF FUNCTIONAL INTERLEUKIN-4 RECEPTORS
    LAHM, H
    SCHNYDER, B
    WYNIGER, J
    BORBENYI, Z
    YILMAZ, A
    CAR, BD
    FISCHER, JR
    GIVEL, JC
    RYFFEL, B
    INTERNATIONAL JOURNAL OF CANCER, 1994, 59 (03) : 440 - 447
12345