Molecular cloning, expression and characterization of the Canis familiaris interleukin-4

被引：13
|
作者：
Wondimu, A
Veit, M
Kohn, B
Kaul, S
Hoffmann, A
Brunnberg, L
Schmidt, MFG
机构：
[1] Free Univ Berlin, Inst Immunol & Mol Biol, D-10115 Berlin, Germany
[2] Free Univ Berlin, Clin Small Anim, D-10115 Berlin, Germany
[3] Paul Ehrlich Inst, D-63225 Langen, Germany
来源：
CYTOKINE | 2001年 / 16卷 / 03期
关键词：
interleukin; 4; canine cytokines; recombinant cytokines; expression vectors;
D O I：
10.1006/cyto.2001.0953
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Interleukin 4 (IL-4) is an important regulatory cytokine produced by activated T lymphocytes and mast cells, and regulates the growth and differentiation of cells such as B and T lymphocytes. The rapid amplification of cDNA ends (RACE) was used to clone the canine IL-4 gene. It was expressed in mammalian cells and Escherichia coli. Monoclonal antibodies were raised to rcIL-4 for use in an enzyme-linked immunosorbent assay (ELISA). This will facilitate studies on the role of cIL-4 in inflammatory diseases, particularly rheumatoid arthritis. (C) 2001 Academic Press.
引用
收藏
页码：88 / 92
页数：5
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