Mapping genetic interactions in human cancer cells with RNAi and multiparametric phenotyping

被引：0
|
作者：
Laufer, Christina [1 ,2 ]
Fischer, Bernd [3 ]
Billmann, Maximilian [1 ,2 ]
Huber, Wolfgang [3 ]
Boutros, Michael [1 ,2 ]
机构：
[1] German Canc Res Ctr, Div Signalling & Funct Gen, Heidelberg, Germany
[2] Heidelberg Univ, Med Fac Mannheim, Dept Cell & Mol Biol, Heidelberg, Germany
[3] European Mol Biol Lab, Genome Biol Unit, Heidelberg, Germany
来源：
NATURE METHODS | 2013年 / 10卷 / 05期
基金：
欧洲研究理事会;
关键词：
GENOME-WIDE RNAI; NETWORKS; PROFILES; REVEALS; SCREENS; COMPLEX; DESIGN; BREAST;
D O I：
10.1038/NMETH.2436
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Genetic interactions influence many phenotypes and can be used as a powerful experimental tool to discover functional relationships between genes. Here we describe a robust and scalable method to systematically map genetic interactions in human cancer cells using combinatorial RNAi and high-throughput imaging. Through automated, single-cell phenotyping, we measured genetic interactions across a broad spectrum of phenotypes, including cell count, cell eccentricity and nuclear area. We mapped genetic interactions of epigenetic regulators in colon cancer cells, recovering known protein complexes. Our study also revealed the prospects and challenges of studying genetic interactions in human cells using multiparametric phenotyping.
引用
收藏
页码：427 / +
页数：7
相关论文
共 50 条
  • [41] CRISPR-based mapping of genetic interactions
    Cloney, Ross
    NATURE REVIEWS GENETICS, 2017, 18 (05) : 272 - 272
  • [42] CRISPR-based mapping of genetic interactions
    Ross Cloney
    Nature Reviews Genetics, 2017, 18 : 272 - 272
  • [43] Mapping reductase enzyme modulation in human colorectal cancer cells
    Erzinger, Melanie M.
    Bovet, Cedric
    Hecht, Katrin M.
    Winiker, Pascale F.
    Sturla, Shana J.
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2012, 244
  • [44] Genetic interactions between BLM and DNA ligase IV in human cells
    So, S
    Adachi, N
    Lieber, MR
    Koyama, H
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (53) : 55433 - 55442
  • [45] Mapping of the human RNASEL promoter and expression in cancer and normal cells
    Zhou, AM
    Molinaro, RJ
    Malathi, K
    Stlverman, RH
    JOURNAL OF INTERFERON AND CYTOKINE RESEARCH, 2005, 25 (10): : 595 - 603
  • [46] RNAi: a potential new class of therapeutic for human genetic disease
    Attila A. Seyhan
    Human Genetics, 2011, 130 : 583 - 605
  • [47] Mapping the Interactions among Biomaterials, Adsorbed Proteins, and Human Embryonic Stem Cells
    Mei, Ying
    Gerecht, Sharon
    Taylor, Michael
    Urquhart, Andrew.
    Bogatyrev, Said R.
    Cho, Seung-Woo
    Davies, Martyn C.
    Alexander, Morgan R.
    Langer, Robert S.
    Anderson, Daniel G.
    ADVANCED MATERIALS, 2009, 21 (27) : 2781 - +
  • [48] RNAi: a potential new class of therapeutic for human genetic disease
    Seyhan, Attila A.
    HUMAN GENETICS, 2011, 130 (05) : 583 - 605
  • [49] Assessing Digital Phenotyping to Enhance Genetic Studies of Human Diseases
    DeBoever, Christopher
    Tanigawa, Yosuke
    Aguirre, Matthew
    McInnes, Greg
    Lavertu, Adam
    Rivas, Manuel A.
    AMERICAN JOURNAL OF HUMAN GENETICS, 2020, 106 (05) : 611 - 622
  • [50] Non-invasive phenotyping of tumor biology in cancer patients by multimodality multiparametric MR and PET imaging
    Metz, Stephan
    Lorenzen, Sylvie
    Nekolla, Stephan
    Schwaiger, Markus
    Beer, Ambros
    JOURNAL OF NUCLEAR MEDICINE, 2010, 51
12345