Mapping genetic interactions in human cancer cells with RNAi and multiparametric phenotyping

被引：0
|
作者：
Laufer, Christina [1 ,2 ]
Fischer, Bernd [3 ]
Billmann, Maximilian [1 ,2 ]
Huber, Wolfgang [3 ]
Boutros, Michael [1 ,2 ]
机构：
[1] German Canc Res Ctr, Div Signalling & Funct Gen, Heidelberg, Germany
[2] Heidelberg Univ, Med Fac Mannheim, Dept Cell & Mol Biol, Heidelberg, Germany
[3] European Mol Biol Lab, Genome Biol Unit, Heidelberg, Germany
来源：
NATURE METHODS | 2013年 / 10卷 / 05期
基金：
欧洲研究理事会;
关键词：
GENOME-WIDE RNAI; NETWORKS; PROFILES; REVEALS; SCREENS; COMPLEX; DESIGN; BREAST;
D O I：
10.1038/NMETH.2436
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Genetic interactions influence many phenotypes and can be used as a powerful experimental tool to discover functional relationships between genes. Here we describe a robust and scalable method to systematically map genetic interactions in human cancer cells using combinatorial RNAi and high-throughput imaging. Through automated, single-cell phenotyping, we measured genetic interactions across a broad spectrum of phenotypes, including cell count, cell eccentricity and nuclear area. We mapped genetic interactions of epigenetic regulators in colon cancer cells, recovering known protein complexes. Our study also revealed the prospects and challenges of studying genetic interactions in human cells using multiparametric phenotyping.
引用
收藏
页码：427 / +
页数：7
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