A System of Photocatalysis for NAD+ Regeneration of Product of (S)-1-Pheylethanol by Enzymic Catalysis

被引:0
|
作者
Pu, Shujin [1 ]
Zhang, Xuan [1 ]
Huang, Han [1 ]
Yang, Chengli [1 ]
Li, Dali [1 ]
Yang, Junfang [1 ]
Ouyang, Jie [1 ]
Chen, Xing [1 ]
Naseer, Sidra [1 ]
Shi, Ruofu [1 ]
机构
[1] Nanjing Univ Sci & Technol, Dept Bioengn, Nanjing 210094, Jiangsu, Peoples R China
关键词
NAD(+); Photocatalysis; Biocatalysis; Riboflavin; (R)-1-phenylethanol dehydrogenase; ALCOHOL-DEHYDROGENASE; NADH REGENERATION; BIOCATALYSIS; OXIDATION;
D O I
10.9767/bcrec.14.2.3930.421-426
中图分类号
TQ [化学工业];
学科分类号
0817 ;
摘要
In this study, a system of photocatalysis for NAD(+) regeneration of enzymatic catalysis was constructed. The optimal conditions for the coupling reaction of photocatalysis and biocatalysis were explored. Blue light was chosen for the efficient reaction and the optimal concentration of VB2 (vitamin B-2, riboflavin) was determined. NAD(+)-dependent (R)-1-phenylethanol dehydrogenase was used in the reaction for transforming (R)-1-phenylethanol to acetophenone. The byproducts of the reaction were just H2O and O-2 by means of catalase. The coupling reaction of catalysis and photocatalysis can be used for obtaining (S)-1-phenylethanol through racemization of 1-phenylethanol. Copyright (c) 2019 BCREC Group. All rights reserved
引用
收藏
页码:421 / 426
页数:6
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