Efficacy of an Adjuvanted Middle East Respiratory Syndrome Coronavirus Spike Protein Vaccine in Dromedary Camels and Alpacas

被引：72

作者：

Adney, Danielle R. ^{[1
]}

Wang, Lingshu ^{[2
]}

van Doremalen, Neeltje ^{[3
]}

Shi, Wei ^{[2
]}

Zhang, Yi ^{[2
]}

Kong, Wing-Pui ^{[2
]}

Miller, Megan R. ^{[3
]}

Bushmaker, Trenton ^{[3
]}

Scott, Dana ^{[3
]}

de Wit, Emmie ^{[3
]}

Modjarrad, Kayvon ^{[4
]}

Petrovsky, Nikolai ^{[5
,6
]}

Graham, Barney S. ^{[2
]}

Bowen, Richard A. ^{[1
]}

Munster, Vincent J. ^{[1
,3
]}

机构：

[1] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80521 USA

[2] NIAID, Vaccine Res Ctr, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA

[3] NIAID, Lab Virol, Div Intramural Res, NIH, Hamilton, MT 59840 USA

[4] Walter Reed Army Inst Res, Mil HIV Res, Silver Spring, MD 20910 USA

[5] Flinders Univ S Australia, Bedford Pk, SA 5042, Australia

[6] Flinders Med Ctr, Vaxine Pty Ltd, Bedford, SA 5042, Australia

来源：

VIRUSES-BASEL | 2019年 / 11卷 / 03期

基金：

美国国家卫生研究院;

关键词：

MERS-CoV; camels; vaccines; One Health; MERS-COV INFECTION; TO-HUMAN TRANSMISSION; HIGH PREVALENCE; SAUDI-ARABIA; ANTIBODIES; REPLICATION; EXPOSURE; RISK;

D O I：

10.3390/v11030212

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

MERS-CoV is present in dromedary camels throughout the Middle East and Africa. Dromedary camels are the primary zoonotic reservoir for human infections. Interruption of the zoonotic transmission chain from camels to humans, therefore, may be an effective strategy to control the ongoing MERS-CoV outbreak. Here we show that vaccination with an adjuvanted MERS-CoV Spike protein subunit vaccine confers complete protection from MERS-CoV disease in alpaca and results in reduced and delayed viral shedding in the upper airways of dromedary camels. Protection in alpaca correlates with high serum neutralizing antibody titers. Lower titers of serum neutralizing antibodies correlate with delayed and significantly reduced shedding in the nasal turbinates of dromedary camels. Together, these data indicate that induction of robust neutralizing humoral immune responses by vaccination of naive animals reduces shedding that potentially could diminish the risk of zoonotic transmission.

引用

页数：13

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