Efficacy of an Adjuvanted Middle East Respiratory Syndrome Coronavirus Spike Protein Vaccine in Dromedary Camels and Alpacas

被引：72

作者：

Adney, Danielle R. ^{[1
]}

Wang, Lingshu ^{[2
]}

van Doremalen, Neeltje ^{[3
]}

Shi, Wei ^{[2
]}

Zhang, Yi ^{[2
]}

Kong, Wing-Pui ^{[2
]}

Miller, Megan R. ^{[3
]}

Bushmaker, Trenton ^{[3
]}

Scott, Dana ^{[3
]}

de Wit, Emmie ^{[3
]}

Modjarrad, Kayvon ^{[4
]}

Petrovsky, Nikolai ^{[5
,6
]}

Graham, Barney S. ^{[2
]}

Bowen, Richard A. ^{[1
]}

Munster, Vincent J. ^{[1
,3
]}

机构：

[1] Colorado State Univ, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80521 USA

[2] NIAID, Vaccine Res Ctr, NIH, 9000 Rockville Pike, Bethesda, MD 20892 USA

[3] NIAID, Lab Virol, Div Intramural Res, NIH, Hamilton, MT 59840 USA

[4] Walter Reed Army Inst Res, Mil HIV Res, Silver Spring, MD 20910 USA

[5] Flinders Univ S Australia, Bedford Pk, SA 5042, Australia

[6] Flinders Med Ctr, Vaxine Pty Ltd, Bedford, SA 5042, Australia

来源：

VIRUSES-BASEL | 2019年 / 11卷 / 03期

基金：

美国国家卫生研究院;

关键词：

MERS-CoV; camels; vaccines; One Health; MERS-COV INFECTION; TO-HUMAN TRANSMISSION; HIGH PREVALENCE; SAUDI-ARABIA; ANTIBODIES; REPLICATION; EXPOSURE; RISK;

D O I：

10.3390/v11030212

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

MERS-CoV is present in dromedary camels throughout the Middle East and Africa. Dromedary camels are the primary zoonotic reservoir for human infections. Interruption of the zoonotic transmission chain from camels to humans, therefore, may be an effective strategy to control the ongoing MERS-CoV outbreak. Here we show that vaccination with an adjuvanted MERS-CoV Spike protein subunit vaccine confers complete protection from MERS-CoV disease in alpaca and results in reduced and delayed viral shedding in the upper airways of dromedary camels. Protection in alpaca correlates with high serum neutralizing antibody titers. Lower titers of serum neutralizing antibodies correlate with delayed and significantly reduced shedding in the nasal turbinates of dromedary camels. Together, these data indicate that induction of robust neutralizing humoral immune responses by vaccination of naive animals reduces shedding that potentially could diminish the risk of zoonotic transmission.

引用

页数：13

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[31] Experimental Infection and Response to Rechallenge of Alpacas with Middle East Respiratory Syndrome Coronavirus
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Riddell, Sarah
Haining, Jessica
Johnson, Dayna
Hemida, Maged G.
Barr, Jennifer
Peiris, Malik
Middleton, Deborah
Wang, Lin-Fa
[J]. EMERGING INFECTIOUS DISEASES, 2016, 22 (06) : 1071 - 1074
[32] Limited Genetic Diversity Detected in Middle East Respiratory Syndrome-Related Coronavirus Variants Circulating in Dromedary Camels in Jordan
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Talafha, Hani A.
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McDowell, Chester
Steel, John
Abu-Basha, Ehab A.
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[33] Epidemiological study of Middle East respiratory syndrome coronavirus infection in dromedary camels in Saudi Arabia, April-May 2015
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Ahmed, A. G.
Abdalla, M. O.
Gumaa, E.
Osman, O. H.
Younis, A. E.
Abu-Obeida, A.
Al-Hafufi, A. N.
Saif, L. J.
Zaki, A.
AI-Rumaihi, A.
Al-Harbi, N.
Kasem, S.
Al-Brahim, R. H.
Ai-Sahhaf, A.
Bayoumi, F. E.
Al-Dowairij, A.
Qasim, I. A.
[J]. REVUE SCIENTIFIQUE ET TECHNIQUE-OFFICE INTERNATIONAL DES EPIZOOTIES, 2018, 37 (03): : 985 - 997
[34] Enzootic patterns of Middle East respiratory syndrome coronavirus in imported African and local Arabian dromedary camels: a prospective genomic study
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Harakeh, Steve M.
Alzahrani, Abdulrahman A.
Alsaaidi, Ghaleb A.
Alagili, Abdulaziz N.
Hashem, Anwar M.
Zumla, Alimuddin
Drosten, Christian
Azhar, Esam I.
[J]. LANCET PLANETARY HEALTH, 2019, 3 (12): : E521 - E528
[35] Middle Eastern Respiratory Syndrome coronavirus and camels
Isaacs, David
[J]. JOURNAL OF PAEDIATRICS AND CHILD HEALTH, 2014, 50 (11) : 929 - 929
[36] Middle East Respiratory Syndrome-Coronavirus Seropositive Bactrian Camels, Mongolia
Bold, Dashzeveg
van Doremalen, Neeltje
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Richt, Juergen A.
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[37] Lack of Middle East Respiratory Syndrome Coronavirus Transmission from Infected Camels
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Al-Naeem, Abdulmohsen
Perera, Ranawaka A. P. M.
Chin, Alex W. H.
Poon, Leo L. M.
Peiris, Malik
[J]. EMERGING INFECTIOUS DISEASES, 2015, 21 (04) : 699 - 701
[38] Systematic, active surveillance for Middle East respiratory syndrome coronavirus in camels in Egypt
Ali, Mohamed A.
Shehata, Mahmoud M.
Gomaa, Mokhtar R.
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Kayed, Ahmed S.
El-Taweel, Ahmed N.
Atea, Mohamed
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Moatasim, Yassmin
Mahmoud, Sara H.
Kutkat, Omnia
Maatouq, Asmaa M.
Osman, Ahmed
McKenzie, Pamela P.
Webby, Richard J.
Kayali, Ghazi
[J]. EMERGING MICROBES & INFECTIONS, 2017, 6
[39] Effect of Fc Fusion on Folding and Immunogenicity of Middle East Respiratory Syndrome Coronavirus Spike Protein
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Park, Ki Hoon
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Lee, Hee-Jung
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[J]. JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, 2019, 29 (05) : 813 - 819
[40] Middle East respiratory syndrome coronavirus Spike protein variants exhibit geographic differences in virulence
Wong, Lok-Yin Roy
Zheng, Jian
Sariol, Alan
Lowery, Shea
Meyerholz, David K.
Gallagher, Tom
Perlman, Stanley
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