Recombinant streptokinase production by fed-batch cultivation of Escherichia coli

被引：15
|
作者：
Zhang, XW [1 ]
Sun, T [1 ]
Huang, XN [1 ]
Liu, X [1 ]
Gu, DX [1 ]
Tang, ZQ [1 ]
机构：
[1] Zhongshan Univ, Food Engn Res Ctr, Guangzhou 510275, Peoples R China
来源：
ENZYME AND MICROBIAL TECHNOLOGY | 1999年 / 24卷 / 10期
关键词：
recombinant; Escherichia coli; streptokinase; fermentation; purification;
D O I：
10.1016/S0141-0229(98)00149-5
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Streptokinase (SK) is a specific effective medicine for thrombolytic therapy of acute myocardial infarction. This study established a process for the pilot production of recombinant streptokinase (r-SK). Engineering bacteria were fermented in a 20-1 fermentor to produce r-SK After simple renaturation and purification, 12.9 g of r-SK with 97.8% of purity and about 10(5) IU mg(-1) of specific activity was obtained, the yield of protein and the recovery of activity were 44.9% and 51%, respectively. Finally, the r-SK was made into about 700 doses of injections for clinical applications. (C) 1999 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：647 / 650
页数：4
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