Changes in γ-H2AX expression in irradiated feline sarcoma cells: An indicator of double strand DNA breaks

被引：7
|
作者：
Lawrence, J. [1 ,2 ]
Karpuzoglu, E. [2 ]
Vance, A. [1 ]
Vandenplas, M. [3 ]
Saba, C. [1 ]
Turek, M. [1 ,2 ]
Gogal, R. M., Jr. [2 ]
机构：
[1] Univ Georgia, Coll Vet Med, Dept Small Anim Med & Surg, Athens, GA 30602 USA
[2] Univ Georgia, Dept Anat & Radiol, Coll Vet Med, Athens, GA 30602 USA
[3] Univ Georgia, Dept Large Anim Med, Coll Vet Med, Athens, GA 30602 USA
来源：
RESEARCH IN VETERINARY SCIENCE | 2013年 / 94卷 / 03期
关键词：
Western Blot; Chemiluminescence; Immunocytochemistry; Tumor; Feline; VACCINE-ASSOCIATED SARCOMAS; CATS; INJECTION; HUMANS;
D O I：
10.1016/j.rvsc.2013.01.007
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Feline injection site sarcoma (ISS) is a highly invasive soft tissue tumor that is commonly treated with radiation. Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The objective of this study was to determine if radiation-induced damage to feline ISS cells could be detected using a commercially available DNA DSB detection kit. Feline ISS cells were irradiated and evaluated for extent of DSB induction with a gamma-H2AX chemiluminescent kit; results were validated by Western Blot analysis. Irradiated cells showed a significant increase in double strand break induction compared to control cells, which was supported by Western Blot. DNA damage in feline sarcoma cells following single exposure of radiation can be indirectly detected using a commercially available mouse anti-human monoclonal antibody for gamma-H2AX. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：545 / 548
页数：4
相关论文
共 50 条
  • [41] The SNF2 family ATPase LSH promotes phosphorylation of H2AX and efficient repair of DNA double-strand breaks in mammalian cells
    Burrage, Joe
    Termanis, Ausma
    Geissner, Andreas
    Myant, Kevin
    Gordon, Katrina
    Stancheva, Irina
    JOURNAL OF CELL SCIENCE, 2012, 125 (22) : 5524 - 5534
  • [42] Histone H2AX phosphorylation as a measure of DNA double-strand breaks and a marker of environmental stress and disease activity in lupus
    Namas, Rajaie
    Renauer, Paul
    Ognenovski, Mikhail
    Tsou, Pei-Suen
    Sawalha, Amr H.
    LUPUS SCIENCE & MEDICINE, 2016, 3 (01):
  • [43] Detection of DNA double-strand breaks using γH2AX after MRI exposure at 3 Tesla:: An in vitro study
    Schwenzer, Nina F.
    Bantlon, Ruediger
    Maurer, Brigitte
    Kehlbach, Rainer
    Schraml, Christina
    Claussen, Claus D.
    Rodegerdts, Enno
    JOURNAL OF MAGNETIC RESONANCE IMAGING, 2007, 26 (05) : 1308 - 1314
  • [44] Factors to Consider for the Correct Use of γH2AX in the Evaluation of DNA Double-Strand Breaks Damage Caused by Ionizing Radiation
    Valente, Davide
    Gentileschi, Maria Pia
    Guerrisi, Antonino
    Bruzzaniti, Vicente
    Morrone, Aldo
    Soddu, Silvia
    Verdina, Alessandra
    CANCERS, 2022, 14 (24)
  • [45] Does γH2AX foci formation depend on the presence of DNA double strand breaks? (vol 229, pg 171, 2005)
    Takahashi, Akihisa
    Ohnishi, Takeo
    CANCER LETTERS, 2006, 236 (01) : 155 - 156
  • [46] Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-Strand Breaks
    Popp, Henning D.
    Brendel, Susanne
    Hofmann, Wolf-Karsten
    Fabarius, Alice
    JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2017, (129):
  • [47] Prediction of clonogenic cell survival curves based on the number of residual DNA double strand breaks measured by γH2AX staining
    Menegakis, Apostolos
    Yaromina, Ala
    Eicheler, Wolfgang
    Doerfler, Annegret
    Beuthien-Baumann, Bettina
    Thames, Howard D.
    Baumann, Michael
    Krause, Mechthild
    INTERNATIONAL JOURNAL OF RADIATION BIOLOGY, 2009, 85 (11) : 1032 - 1041
  • [48] Immunohistochemical analysis of γH2AX, a biomarker for DNA double-strand breaks, in the liver of genotoxic carcinogen-treated mice
    Abe, M.
    Hagio, S.
    Tsuji, N.
    Kuroda, Y.
    Hayashi, S.
    Furukawa, S.
    TOXICOLOGY LETTERS, 2015, 238 (02) : S261 - S262
  • [49] RhoB Promotes γH2AX Dephosphorylation and DNA Double-Strand Break Repair
    Mamouni, Kenza
    Cristini, Agnese
    Guirouilh-Barbat, Josee
    Monferran, Sylvie
    Lemarie, Anthony
    Faye, Jean-Charles
    Lopez, Bernard S.
    Favre, Gilles
    Sordet, Olivier
    MOLECULAR AND CELLULAR BIOLOGY, 2014, 34 (16) : 3144 - 3155
  • [50] H2AX prevents DNA breaks from progressing to chromosome breaks and translocations
    Franco, S
    Gostissa, M
    Zha, S
    Lombard, DB
    Murphy, MM
    Zarrin, AA
    Yan, C
    Tepsuporn, S
    Morales, JC
    Adams, MM
    Lou, ZK
    Bassing, CH
    Manis, JP
    Chen, JJ
    Carpenter, PB
    Alt, FW
    MOLECULAR CELL, 2006, 21 (02) : 201 - 214
12345