Changes in γ-H2AX expression in irradiated feline sarcoma cells: An indicator of double strand DNA breaks

被引：7
|
作者：
Lawrence, J. [1 ,2 ]
Karpuzoglu, E. [2 ]
Vance, A. [1 ]
Vandenplas, M. [3 ]
Saba, C. [1 ]
Turek, M. [1 ,2 ]
Gogal, R. M., Jr. [2 ]
机构：
[1] Univ Georgia, Coll Vet Med, Dept Small Anim Med & Surg, Athens, GA 30602 USA
[2] Univ Georgia, Dept Anat & Radiol, Coll Vet Med, Athens, GA 30602 USA
[3] Univ Georgia, Dept Large Anim Med, Coll Vet Med, Athens, GA 30602 USA
来源：
RESEARCH IN VETERINARY SCIENCE | 2013年 / 94卷 / 03期
关键词：
Western Blot; Chemiluminescence; Immunocytochemistry; Tumor; Feline; VACCINE-ASSOCIATED SARCOMAS; CATS; INJECTION; HUMANS;
D O I：
10.1016/j.rvsc.2013.01.007
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Feline injection site sarcoma (ISS) is a highly invasive soft tissue tumor that is commonly treated with radiation. Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The objective of this study was to determine if radiation-induced damage to feline ISS cells could be detected using a commercially available DNA DSB detection kit. Feline ISS cells were irradiated and evaluated for extent of DSB induction with a gamma-H2AX chemiluminescent kit; results were validated by Western Blot analysis. Irradiated cells showed a significant increase in double strand break induction compared to control cells, which was supported by Western Blot. DNA damage in feline sarcoma cells following single exposure of radiation can be indirectly detected using a commercially available mouse anti-human monoclonal antibody for gamma-H2AX. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：545 / 548
页数：4
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