Changes in γ-H2AX expression in irradiated feline sarcoma cells: An indicator of double strand DNA breaks

被引：7
|
作者：
Lawrence, J. [1 ,2 ]
Karpuzoglu, E. [2 ]
Vance, A. [1 ]
Vandenplas, M. [3 ]
Saba, C. [1 ]
Turek, M. [1 ,2 ]
Gogal, R. M., Jr. [2 ]
机构：
[1] Univ Georgia, Coll Vet Med, Dept Small Anim Med & Surg, Athens, GA 30602 USA
[2] Univ Georgia, Dept Anat & Radiol, Coll Vet Med, Athens, GA 30602 USA
[3] Univ Georgia, Dept Large Anim Med, Coll Vet Med, Athens, GA 30602 USA
来源：
RESEARCH IN VETERINARY SCIENCE | 2013年 / 94卷 / 03期
关键词：
Western Blot; Chemiluminescence; Immunocytochemistry; Tumor; Feline; VACCINE-ASSOCIATED SARCOMAS; CATS; INJECTION; HUMANS;
D O I：
10.1016/j.rvsc.2013.01.007
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Feline injection site sarcoma (ISS) is a highly invasive soft tissue tumor that is commonly treated with radiation. Cellular deoxyribonucleic acid (DNA) is the principal target for the biologic effects of radiation with cell killing correlating to the number of double stranded DNA breaks (DSBs). The objective of this study was to determine if radiation-induced damage to feline ISS cells could be detected using a commercially available DNA DSB detection kit. Feline ISS cells were irradiated and evaluated for extent of DSB induction with a gamma-H2AX chemiluminescent kit; results were validated by Western Blot analysis. Irradiated cells showed a significant increase in double strand break induction compared to control cells, which was supported by Western Blot. DNA damage in feline sarcoma cells following single exposure of radiation can be indirectly detected using a commercially available mouse anti-human monoclonal antibody for gamma-H2AX. (C) 2013 Elsevier Ltd. All rights reserved.
引用
收藏
页码：545 / 548
页数：4
相关论文
共 50 条
  • [1] γ-H2AX as a sensitive indicator of DNA double-strand breaks
    Olive, Peggy L.
    RADIATION RESEARCH, 2007, 167 (03) : 346 - 347
  • [2] γH2AX in the recognition of DNA double-strand breaks
    Podhorecka, Monika
    POSTEPY HIGIENY I MEDYCYNY DOSWIADCZALNEJ, 2009, 63 : 92 - 98
  • [3] γ-H2AX -: A novel biomarker for DNA double-strand breaks
    Kuo, Linda J.
    Yang, Li-Xi
    IN VIVO, 2008, 22 (03): : 305 - 309
  • [4] Phosphorylated H2Ax is not an unambiguous marker for DNA double strand breaks
    Cleaver, James E.
    Feeney, Luzviminda
    Revet, Ingrid
    CELL CYCLE, 2011, 10 (19) : 3223 - 3224
  • [5] Characteristics of γ-H2AX foci at DNA double strand breaks sites
    Pilch, DR
    Sedelnikova, OA
    Redon, C
    Celeste, A
    Nussenzweig, A
    Bonner, WM
    BIOCHEMISTRY AND CELL BIOLOGY, 2003, 81 (03) : 123 - 129
  • [6] γ-H2AX as a biomarker for DNA double-strand breaks in ecotoxicology
    Geric, Marko
    Gajski, Goran
    Garaj-Vrhovac, Vera
    ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2014, 105 : 13 - 21
  • [7] DNA double-strand breaks and γ-H2AX signaling in the testis
    Hamer, G
    Roepers-Gajadien, HL
    van Duyn-Goedhart, A
    Gademan, IS
    Kal, HB
    van Buul, PPW
    de Rooij, DG
    BIOLOGY OF REPRODUCTION, 2003, 68 (02) : 628 - 634
  • [8] INDUCTION AND REJOINING OF DNA DOUBLE STRAND BREAKS ASSESSED BY H2AX PHOSPHORYLATION IN MELANOMA CELLS IRRADIATED WITH PROTON AND LITHIUM BEAMS
    Ibanez, Irene L.
    Bracalente, Candelaria
    Molinari, Beatriz L.
    Palmieri, Monica A.
    Policastro, Lucia
    Kreiner, Andres J.
    Burlon, Alejandro A.
    Valda, Alejandro
    Navalesi, Daniela
    Davidson, Jorge
    Davidson, Miguel
    Vazquez, Monica
    Ozafran, Mabel
    Duran, Hebe
    INTERNATIONAL JOURNAL OF RADIATION ONCOLOGY BIOLOGY PHYSICS, 2009, 74 (04): : 1226 - 1235
  • [9] Low level phosphorylation of histone H2AX on serine 139 (γH2AX) is not associated with DNA double-strand breaks
    Rybak, Paulina
    Hoang, Agnieszka
    Bujnowicz, Lukasz
    Bernas, Tytus
    Berniak, Krzysztof
    Zarebski, Miroslaw
    Darzynkiewicz, Zbigniew
    Dobrucki, Jerzy
    ONCOTARGET, 2016, 7 (31) : 49574 - 49587
  • [10] H2AX phosphorylation at the sites of DNA double-strand breaks in cultivated mammalian cells and tissues
    Firsanov, Denis V.
    Solovjeva, Liudmila V.
    Svetlova, Maria P.
    CLINICAL EPIGENETICS, 2011, 2 : 283 - 297
12345