Enhancer competition between H19 and Igf2 does not mediate their imprinting

被引：89
|
作者：
Schmidt, JV
Levorse, JM
Tilghman, SM
机构：
[1] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA
[2] Princeton Univ, Howard Hughes Med Inst, Princeton, NJ 08544 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1999年 / 96卷 / 17期
关键词：
D O I：
10.1073/pnas.96.17.9733
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The linked H19 and Igf2 genes on mouse distal chromosome 7 are subject to genomic imprinting. Competition between the promoters of the genes for transcription from shared enhancers has been proposed as an explanation for the coordinate expression and reciprocal imprinting of these two genes, To test this model, we have used Cre-loxP technology to generate in mice a conditional deletion of the H19 promoter and structural gene that leaves no transcription unit in the locus. Contrary to the prediction of enhancer competition we find that transcriptional activity from the H19 promoter is not required for the imprinted silencing of the Igf2 gene.
引用
收藏
页码：9733 / 9738
页数：6
相关论文
共 50 条
  • [41] Embryo vitrification affects the methylation of the H19/Igf2 differentially methylated domain and the expression of H19 and Igf2
    Wang, Zengyan
    Xu, Ling
    He, Fangfang
    FERTILITY AND STERILITY, 2010, 93 (08) : 2729 - 2733
  • [42] Paternal bias expression of Igf2as is enhancer-dependent on the imprinting cluster of Igf2, H19 and Nctc1 in muscle cells
    Kim, Jae-Young
    Youn, Hwa Young
    Pfeifer, Karl
    Eun, Bokkee
    ANIMAL CELLS AND SYSTEMS, 2019, 23 (04) : 288 - 293
  • [43] Frequent loss of imprinting at the IGF2 and H19 genes in head and neck squamous carcinoma
    Adel K El-Naggar
    Syeling Lai
    Susan A Tucker
    Gary L Clayman
    Helmuth Goepfert
    Waun Ki Hong
    Vicki Huff
    Oncogene, 1999, 18 : 7063 - 7069
  • [44] Loss of imprinting of IGF2 correlates with hypermethylation of the H19 differentially methylated region in hepatoblastoma
    S Honda
    Y Arai
    M Haruta
    F Sasaki
    M Ohira
    H Yamaoka
    H Horie
    A Nakagawara
    E Hiyama
    S Todo
    Y Kaneko
    British Journal of Cancer, 2008, 99 : 1891 - 1899
  • [45] Frequent loss of imprinting at the IGF2 and H19 genes in head and neck squamous carcinoma
    El-Naggar, AK
    Lai, SL
    Tucker, SA
    Clayman, GL
    Goepfert, H
    Hong, WK
    Huff, V
    ONCOGENE, 1999, 18 (50) : 7063 - 7069
  • [46] Frequent loss of imprinting at the IGF2 and H19 genes in head and neck squamous carcinoma
    El-Naggar, AK
    Lai, S
    Clayman, GL
    Luna, MA
    LABORATORY INVESTIGATION, 1999, 79 (01) : 174A - 174A
  • [47] Imprinting of IGF2 and H19: Lack of reciprocity in sporadic Beckwith-Wiedemann syndrome
    Joyce, JA
    Lam, WK
    Catchpoole, DJ
    Jenks, P
    Reik, W
    Maher, ER
    Schofield, PN
    HUMAN MOLECULAR GENETICS, 1997, 6 (09) : 1543 - 1548
  • [48] Loss of imprinting of IGF2 correlates with hypermethylation of the H19 differentially methylated region in hepatoblastoma
    Honda, S.
    Arai, Y.
    Haruta, M.
    Sasaki, F.
    Ohira, M.
    Yamaoka, H.
    Horie, H.
    Nakagawara, A.
    Hiyama, E.
    Todo, S.
    Kaneko, Y.
    BRITISH JOURNAL OF CANCER, 2008, 99 (11) : 1891 - 1899
  • [49] EPIGENETIC REGULATION OF THE IGF2/H19 LOCUS
    Engstrom, Wilhelm
    Nordin, Matilda
    Bergman, Daniel
    Halje, Matilda
    Laestander, Caroline
    Andersson, Erika
    Lindgren, Michelle Alexius
    Lindstedt, Ingrid
    ANTICANCER RESEARCH, 2014, 34 (10) : 5895 - 5895
  • [50] CTCF binding at the insulin-like growth factor-II (IGF2)/H19 imprinting control region is insufficient to regulate IGF2/H19 expression in human tissues
    Ulaner, GA
    Yang, YW
    Hu, JF
    Li, T
    Vu, TH
    Hoffman, AR
    ENDOCRINOLOGY, 2003, 144 (10) : 4420 - 4426
12345