Enhancer competition between H19 and Igf2 does not mediate their imprinting

被引：89
|
作者：
Schmidt, JV
Levorse, JM
Tilghman, SM
机构：
[1] Princeton Univ, Dept Mol Biol, Princeton, NJ 08544 USA
[2] Princeton Univ, Howard Hughes Med Inst, Princeton, NJ 08544 USA
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1999年 / 96卷 / 17期
关键词：
D O I：
10.1073/pnas.96.17.9733
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The linked H19 and Igf2 genes on mouse distal chromosome 7 are subject to genomic imprinting. Competition between the promoters of the genes for transcription from shared enhancers has been proposed as an explanation for the coordinate expression and reciprocal imprinting of these two genes, To test this model, we have used Cre-loxP technology to generate in mice a conditional deletion of the H19 promoter and structural gene that leaves no transcription unit in the locus. Contrary to the prediction of enhancer competition we find that transcriptional activity from the H19 promoter is not required for the imprinted silencing of the Igf2 gene.
引用
收藏
页码：9733 / 9738
页数：6
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