Isolation and characterization of 24 polymorphic microsatellite loci in Sepioteuthis lessoniana

被引：3
|
作者：
Zheng, X. -D. [1 ,2 ]
Zuo, Z. -R. [1 ]
Su, X. -J. [3 ]
Li, Q. [1 ]
机构：
[1] Ocean Univ China, Coll Fisheries, Qingdao, Peoples R China
[2] Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao, Peoples R China
[3] Guangxi Univ, Coll Anim Sci & Technol, Nanning 530004, Guangxi, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2012年 / 11卷 / 04期
基金：
国家高技术研究发展计划(863计划);
关键词：
Microsatellite; Cephalopoda; Sepioteuthis lessoniana; Enriched genomic library; COMPLEX;
D O I：
10.4238/2012.November.14.3
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Owing to their codominant, multiallelic, and highly polymorphic nature, microsatellite markers have been used widely in population genetics and biological resource conservation studies. To investigate the genetic structure of Sepioteuthis lessoniana, we developed 24 microsatellite DNA markers and assessed the polymorphism of each locus in a wild S. lessoniana population. The number of alleles per locus ranged from 4 to 26, and the observed and expected heterozygosities varied from 0.188 to 1.000 and 0.392 to 0.959 with an average of 0.675 and 0.852, respectively. These microsatellite loci will be useful tools in future studies of population genetic structure in this species.
引用
收藏
页码：3961 / 3965
页数：5
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