Isolation and characterization of 20 polymorphic microsatellite loci for Scaptodrosophila hibisci

被引：13
|
作者：
Wilson, ACC
Sunnucks, P
Barker, JSF
机构：
[1] Macquarie Univ, Dept Sci Biol, N Ryde, NSW 2109, Australia
[2] Univ New England, Sch Rural Sci & Nat Resources, Armidale, NSW 2351, Australia
来源：
MOLECULAR ECOLOGY NOTES | 2002年 / 2卷 / 03期
关键词：
Drosophilidae; juxtaposed loci; null alleles; segregation distortion; sex linkage; X-linkage;
D O I：
10.1046/j.1471-8286.2002.00212.x
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Scaptodrosophila hibisci is an endemic Australian Drosophilidae that breeds in the flowers of native Hibiscus. Here we report the isolation and amplification of 20 polymorphic microsatellite loci. We cloned these microsatellites because loci developed for Drosophila melanogaster failed to amplify in S. hibisci. Null alleles were detected at six loci, and five were X-linked. Two of the primer pairs amplified an unlinked 'bonus' locus. One locus containing juxtaposed microsatellite loci was suitable for designing an additional set of primers. Mean number of alleles per locus was 10, mean H-O and H-E per locus were 0.532 and 0.636, respectively.
引用
收藏
页码：242 / 244
页数：3
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