SPECTROPHOTOMETRIC AND SPECTROFLUORIMETRIC DETERMINATION OF 1,4-DIHYDROPYRIDINE DRUGS USING POTASSIUM PERMANGANATE AND CERIUM (IV) AMMONIUM SULPHATE

Simple and sensitive spectrophotometric and spectrofluorimetric methods have been developed for determination of 1,4-dihydropyridine (1,4-DHP) drugs based on the oxidation of the investigated 1,4-DHP drugs with acidic KMnO4 (method I) or Ce (IV) (method II). The first method is based on the decrease in the colour of the permanganate solution due to the presence of the studied drug was measured at 525 nm. And the second method is based on monitoring the fluorescence of the produced cerium (III) at emission 355 nm (excitation at 255 nm). All variables that affect the performance of the proposed methods were carefully studied and optimized. The analytical performance of the methods was validated according to International Conference of Harmonization guidelines. The proposed methods were applied successfully to the determination of the drugs in commercial tablets and capsules. The results of the proposed procedures were statistically and compared with those obtained by the reference methods.