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A hybrid bacterial replication origin
被引:4
|作者:
Seitz, H
[1
]
Welzeck, M
[1
]
Messer, W
[1
]
机构:
[1] Max Planck Inst Mol Genet, D-14195 Berlin, Germany
来源:
关键词:
D O I:
10.1093/embo-reports/kve225
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
We constructed a hybrid replication origin that consists of the main part of oriC from Escherichia coli, the DnaA box region and the AT-rich region from Bacillus subtilis oriC. The AT-rich region could be unwound by E. coli DnaA protein, and the DnaB helicase was loaded into the single-stranded bubble. The results show that species specificity, i.e. which DnaA protein can do the unwinding, resides within the DnaA box region of oriC.
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页码:1003 / 1006
页数:4
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