A hybrid bacterial replication origin

被引：4
|
作者：
Seitz, H [1 ]
Welzeck, M [1 ]
Messer, W [1 ]
机构：
[1] Max Planck Inst Mol Genet, D-14195 Berlin, Germany
来源：
EMBO REPORTS | 2001年 / 2卷 / 11期
关键词：
D O I：
10.1093/embo-reports/kve225
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We constructed a hybrid replication origin that consists of the main part of oriC from Escherichia coli, the DnaA box region and the AT-rich region from Bacillus subtilis oriC. The AT-rich region could be unwound by E. coli DnaA protein, and the DnaB helicase was loaded into the single-stranded bubble. The results show that species specificity, i.e. which DnaA protein can do the unwinding, resides within the DnaA box region of oriC.
引用
收藏
页码：1003 / 1006
页数：4
相关论文
共 50 条
  • [11] A study on the correlation of nucleotide skews and the positioning of the origin of replication: different modes of replication in bacterial species
    Nikolaou, C
    Almirantis, Y
    NUCLEIC ACIDS RESEARCH, 2005, 33 (21) : 6816 - 6822
  • [12] Decoupling Growth and Production by Removing the Origin of Replication from a Bacterial Chromosome
    Kasari, Marje
    Kasari, Villu
    Karmas, Mirjam
    Joers, Arvi
    ACS SYNTHETIC BIOLOGY, 2022, 11 (08): : 2610 - 2622
  • [13] DISCOVERY OF A TRANSCRIPTION TERMINATOR WITHIN THE BACTERIAL ORIGIN OF DNA-REPLICATION
    GANEA, D
    ZYSKIND, JW
    DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1984, 3 (01): : 113 - 113
  • [14] Control of bacterial chromosome replication by non-coding regions outside the origin
    Jakob Frimodt-Møller
    Godefroid Charbon
    Anders Løbner-Olesen
    Current Genetics, 2017, 63 : 607 - 611
  • [15] Chromosomal origin of replication coordinates logically distinct types of bacterial genetic regulation
    Kosmas Kosmidis
    Kim Philipp Jablonski
    Georgi Muskhelishvili
    Marc-Thorsten Hütt
    npj Systems Biology and Applications, 6
  • [16] Control of bacterial chromosome replication by non-coding regions outside the origin
    Frimodt-Moller, Jakob
    Charbon, Godefroid
    Lobner-Olesen, Anders
    CURRENT GENETICS, 2017, 63 (04) : 607 - 611
  • [17] Building a bacterial orisome: emergence of new regulatory features for replication origin unwinding
    Leonard, AC
    Grimwade, JE
    MOLECULAR MICROBIOLOGY, 2005, 55 (04) : 978 - 985
  • [18] Chromosomal origin of replication coordinates logically distinct types of bacterial genetic regulation
    Kosmidis, Kosmas
    Jablonski, Kim Philipp
    Muskhelishvili, Georgi
    Huett, Marc-Thorsten
    NPJ SYSTEMS BIOLOGY AND APPLICATIONS, 2020, 6 (01)
  • [19] Computational prediction of origin of replication in bacterial genomes using correlated entropy measure (CEM)
    Parikh, Harsh
    Singh, Apoorvi
    Krishnamachari, Annangarachari
    Shah, Kushal
    BIOSYSTEMS, 2015, 128 : 19 - 25
  • [20] The Consequences of Replicating in the Wrong Orientation: Bacterial Chromosome Duplication without an Active Replication Origin
    Dimude, Juachi U.
    Stockum, Anna
    Midgley-Smith, Sarah L.
    Upton, Amy L.
    Foster, Helen A.
    Khan, Arshad
    Saunders, Nigel J.
    Retkute, Renata
    Rudolph, Christian J.
    MBIO, 2015, 6 (06):
12345