A hybrid bacterial replication origin

被引：4
|
作者：
Seitz, H [1 ]
Welzeck, M [1 ]
Messer, W [1 ]
机构：
[1] Max Planck Inst Mol Genet, D-14195 Berlin, Germany
来源：
EMBO REPORTS | 2001年 / 2卷 / 11期
关键词：
D O I：
10.1093/embo-reports/kve225
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We constructed a hybrid replication origin that consists of the main part of oriC from Escherichia coli, the DnaA box region and the AT-rich region from Bacillus subtilis oriC. The AT-rich region could be unwound by E. coli DnaA protein, and the DnaB helicase was loaded into the single-stranded bubble. The results show that species specificity, i.e. which DnaA protein can do the unwinding, resides within the DnaA box region of oriC.
引用
收藏
页码：1003 / 1006
页数：4
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