Gel Filtration Chromatography (Size Exclusion Chromatography) of Proteins

被引：29

作者：

Duong-Ly, Krisna C. ^{[1
]}

Gabelli, Sandra B. ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Biophys & Biophys Chem, Baltimore, MD 21205 USA

来源：

LABORATORY METHODS IN ENZYMOLOGY: PROTEIN, PT C | 2014年 / 541卷

关键词：

D O I：

10.1016/B978-0-12-420119-4.00009-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The protocol described here allows the student to construct a standard curve for a gel filtration column with a separation range of 5-250 kD. The size (hydrodynamic radius) of a protein species stable in a buffer containing Tris HCl, NaCl, and DTT is determined using this column. Modifications may be made to the buffer to accommodate the protein of interest and the separation range of the column.

引用

页码：105 / 114

页数：10

共 50 条

[41] SIZE-EXCLUSION CHROMATOGRAPHY OF DNA RESTRICTION FRAGMENTS - FRAGMENT LENGTH DETERMINATIONS AND A COMPARISON WITH THE BEHAVIOR OF PROTEINS IN SIZE-EXCLUSION CHROMATOGRAPHY
ELLEGREN, H
LAAS, T
[J]. JOURNAL OF CHROMATOGRAPHY, 1989, 467 (01): : 217 - 226
[42] Application of HPLC, Gel Electrophoresis and Size Exclusion Chromatography for Separation and Characterization of Storage Proteins of Soybean and Peanut
Bhushan, R.
Dixit, S.
[J]. ACTA CHROMATOGRAPHICA, 2012, 24 (03) : 323 - 349
[43] AQUEOUS SIZE EXCLUSION CHROMATOGRAPHY
ROLLINGS, JE
BOSE, A
CARUTHERS, JM
TSAO, GT
OKOS, MR
[J]. ADVANCES IN CHEMISTRY SERIES, 1983, (203): : 345 - 360
[44] SIZE-EXCLUSION CHROMATOGRAPHY
BARTH, HG
BOYES, BE
JACKSON, C
[J]. ANALYTICAL CHEMISTRY, 1994, 66 (12) : R595 - R620
[45] NOMENCLATURE FOR SIZE EXCLUSION CHROMATOGRAPHY
MORI, S
[J]. ANALYTICAL CHEMISTRY, 1978, 50 (04) : A424 - &
[46] CONTINUOUS SIZE EXCLUSION CHROMATOGRAPHY
BALTUS, RE
DALVIE, SK
[J]. ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1988, 195 : 227 - IEC
[47] SIZE EXCLUSION CHROMATOGRAPHY OF POLYAMIDES
MAROT, G
LESEC, J
[J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1988, 11 (16): : 3305 - 3319
[48] CHEMOMETRICS IN SIZE EXCLUSION CHROMATOGRAPHY
BALKE, ST
[J]. ACS SYMPOSIUM SERIES, 1987, 352 : 202 - 219
[49] Tandem size exclusion chromatography polyacrylamide gel electrophoresis of humic acids
Trubetskoj, OA
Trubetskaya, OE
Afanasieva, GV
Reznikova, OI
Hermosin, B
Saiz-Jimenez, C
[J]. ZEITSCHRIFT FUR PFLANZENERNAHRUNG UND BODENKUNDE, 1998, 161 (06): : 619 - 625
[50] STANDARD PRACTICE OF LIQUID SIZE-EXCLUSION CHROMATOGRAPHY - SEC (GEL-PERMEATION CHROMATOGRAPHY - GPC)
BLY, DD
[J]. JOURNAL OF LIQUID CHROMATOGRAPHY, 1980, 3 (03): : 465 - 470

← 1 2 3 4 5 →