Gel Filtration Chromatography (Size Exclusion Chromatography) of Proteins

被引：29
|
作者：
Duong-Ly, Krisna C. [1 ]
Gabelli, Sandra B. [1 ]
机构：
[1] Johns Hopkins Univ, Sch Med, Dept Biophys & Biophys Chem, Baltimore, MD 21205 USA
来源：
LABORATORY METHODS IN ENZYMOLOGY: PROTEIN, PT C | 2014年 / 541卷
关键词：
D O I：
10.1016/B978-0-12-420119-4.00009-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The protocol described here allows the student to construct a standard curve for a gel filtration column with a separation range of 5-250 kD. The size (hydrodynamic radius) of a protein species stable in a buffer containing Tris HCl, NaCl, and DTT is determined using this column. Modifications may be made to the buffer to accommodate the protein of interest and the separation range of the column.
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页码：105 / 114
页数：10
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