Immunocytochemical localization of the high-affinity glutamate transporter, EAACl, in the retina of representative vertebrate species

被引：39
|
作者：
Schulz, K
Stell, WK
机构：
[1] UNIV CALGARY,FAC MED,LIONS SIGHT CTR,CALGARY,AB T2N 4N1,CANADA
[2] UNIV CALGARY,FAC MED,NEUROSCI RES GRP,CALGARY,AB T2N 4N1,CANADA
[3] UNIV CALGARY,FAC MED,DEPT ANAT,CALGARY,AB T2N 4N1,CANADA
[4] UNIV CALGARY,FAC MED,DEPT SURG OPHTHALMOL,CALGARY,AB T2N 4N1,CANADA
来源：
NEUROSCIENCE LETTERS | 1996年 / 211卷 / 03期
关键词：
glutamate transporter; EAAC1; retina; goldfish; salamander; turtle; chicken; rat; immunocytochemistry;
D O I：
10.1016/0304-3940(96)12762-2
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
The glutamate transporter, EAAC1, was localized immunocytochemically in goldfish, salamander, turtle, chicken, and rat retinas, using affinity-purified oligopeptide antibodies. Immunoreactive (IR) EAAC1 was present in the inner plexiform layer of all species, and in cell bodies of bipolar, amacrine, and ganglion cells of most species, but absent from photoreceptors and Muller's glial cells. Western blots revealed an IR-EAAC1 band at 70 kDa. Staining was abolished by preabsorption with EAAC1 peptide.
引用
收藏
页码：191 / 194
页数：4
相关论文
共 50 条
  • [41] Amyolid precursor protein mediates presynaptic localization and activity of the high-affinity choline transporter
    Wang, Baiping
    Yang, Li
    Wang, Zilai
    Zheng, Hui
    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (35) : 14140 - 14145
  • [42] TIME COURSE OF THE AGING OF THE HIGH-AFFINITY L-GLUTAMATE TRANSPORTER IN RAT CORTICAL SYNAPTOSOMES
    WHEELER, DD
    ONDO, JG
    EXPERIMENTAL GERONTOLOGY, 1986, 21 (03) : 159 - 168
  • [43] Molecular cloning and functional expression of an insect high-affinity Na+-dependent glutamate transporter
    Donly, BC
    Richman, A
    Hawkins, E
    McLean, H
    Caveney, S
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1997, 248 (02): : 535 - 542
  • [44] Inhibition of the high-affinity brain glutamate transporter GLAST-1 via direct phosphorylation
    Conradt, M
    Stoffel, W
    JOURNAL OF NEUROCHEMISTRY, 1997, 68 (03) : 1244 - 1251
  • [45] Cellular distribution of a high-affinity glutamate transporter in the nervous system of the cabbage looper Trichoplusia ni
    Gardiner, RB
    Ullensvang, K
    Danbolt, NC
    Caveney, S
    Donly, BC
    JOURNAL OF EXPERIMENTAL BIOLOGY, 2002, 205 (17): : 2605 - 2614
  • [46] Identification and characterization of the high-affinity choline transporter
    Takashi Okuda
    Tatsuya Haga
    Yoshikatsu Kanai
    Hitoshi Endou
    Takeshi Ishihara
    Isao Katsura
    Nature Neuroscience, 2000, 3 : 120 - 125
  • [47] Structure and function of the high-affinity choline transporter
    Haga, T.
    Osawa, C.
    Nishiyama, N.
    Yamada, H.
    Hayashi, K.
    Nakamura, T.
    Hamano, T.
    Nishikawa, S.
    Ushio, T.
    Kubo, Y.
    Urashima, K.
    Kaitsuka, C.
    Okuda, T.
    JOURNAL OF NEUROCHEMISTRY, 2007, 102 : 172 - 172
  • [48] PURIFICATION AND RECONSTITUTION OF THE HIGH-AFFINITY CHOLINE TRANSPORTER
    KNIPPER, M
    KAHLE, C
    BREER, H
    BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1065 (02) : 107 - 113
  • [49] Identification and characterization of the high-affinity choline transporter
    Okuda, T
    Haga, T
    Kanai, Y
    Endou, H
    Ishihara, T
    Katsura, I
    NATURE NEUROSCIENCE, 2000, 3 (02) : 120 - 125
  • [50] Stress modulates the dopamine high-affinity transporter
    Hadjiconstantinou, M
    Copeland, BJ
    Neff, NH
    JOURNAL OF NEUROCHEMISTRY, 1997, 69 : S61 - S61
12345