Enzymatic synthesis of peptides on a solid support

被引：14
|
作者：
Haddoub, Rose
Dauner, Martin
Stefanowicz, Fiona A.
Barattini, Valeria
Laurent, Nicolas
Flitsch, Sabine L. [1 ]
机构：
[1] Univ Manchester, Manchester Interdisciplinary Bioctr, Manchester M1 7DN, Lancs, England
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2009年 / 7卷 / 04期
基金：
英国惠康基金; 英国生物技术与生命科学研究理事会;
关键词：
SELF-ASSEMBLED MONOLAYERS; MASS-SPECTROMETRY; PROTEASE; GLYCOSYLATION;
D O I：
10.1039/b816847d
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
We have previously shown that dipeptides can be synthesised in high yields from amino acids using protease catalysis in aqueous media, if the amino component is immobilised on porous PEGA resin (a copolymer of polyethylene glycol and polyacrylamide). Here we explore the scope of this methodology for using protected and glycosylated amino acids as well as the synthesis of longer peptides on resin and show that such a method can also be applied on non-porous surfaces, in particular on gold.
引用
收藏
页码：665 / 670
页数：6
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