GADH screen-printed biosensor for gluconic acid determination in wine samples

被引：11
|
作者：
del Torno-de Roman, Lorena [1 ]
Asuncion Alonso-Lomillo, M. [1 ]
Dominguez-Renedo, Olga [1 ]
Jaureguibeitia, Arrate [2 ]
Julia Arcos-Martinez, M. [1 ]
机构：
[1] Univ Burgos, Fac Sci, Dept Analyt Chem, Burgos 09001, Spain
[2] Biolan Microbiosensores SL, Parque Tecnol Vizcaya, Zamudio 48170, Spain
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2014年 / 192卷
关键词：
Screen-printed carbon electrodes; Chronoamperometric biosensor; Gluconate dehydrogenase; Tetrathiafulvalene; Gluconic acid; CAPILLARY-ZONE-ELECTROPHORESIS; ORGANIC-ACIDS; HPLC DETERMINATION; CARBOXYLIC-ACIDS; SUGAR ACIDS; QUANTIFICATION; CHROMATOGRAPHY; SPECTROSCOPY; ELECTRODES; PARAMETERS;
D O I：
10.1016/j.snb.2013.10.098
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Gluconate dehydrogenase (GADH) biosensors have been developed for the satisfactory determination of gluconic acid in wine samples, without any pretreatment. The biosensors have been fabricated by cross-linking immobilization of GADH onto screen-printed carbon electrodes, containing the mediator tetrathiafulvalene (TTF). Chronoamperograms have been registered at +100 mV vs. screen-printed Ag/AgCl electrode by successive additions of a gluconic acid solution in the concentration range from 9.0 to 131.4 mu M. This method shows a reproducibility of 8.1% (n = 3) related to the slopes of these calibration curves and a repeatability of 3.2% (n = 5). The procedure has shown an average capability of detection of 9.0 mu M for a probability of false positive and negative of 0.05. Wine samples have been analyzed with these biosensors, obtaining satisfactory results. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：56 / 59
页数：4
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