PREPARATIVE ISOLATION AND PURIFICATION OF LUTONARIN AND SAPONARIN FROM BARLEY SEEDLINGS BY HSCCC

被引：4

作者：

Chen, Tao ^{[1
,2
]}

Wang, Ping ^{[1
,2
]}

Du, Yuzhi ^{[1
]}

Shen, Yuhu ^{[1
]}

Li, Yulin ^{[1
]}

机构：

[1] Chinese Acad Sci, NW Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining, Qinghai Prov, Peoples R China

[2] Chinese Acad Sci, Grad Sch, Beijing, Peoples R China

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2012年 / 35卷 / 18期

基金：

中国国家自然科学基金;

关键词：

barley seedlings; Hordeum vulgare; HPLC; HSCCC; lutonarin; saponarin; SPEED COUNTERCURRENT CHROMATOGRAPHY; LIQUID-CHROMATOGRAPHY; HORDEUM-VULGARE; FLAVONOIDS; LEAVES;

D O I：

10.1080/10826076.2011.636471

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to isolate large amounts of pure lutonarin and saponarin, a high performance method for isolation and purification of these two flavonoids from barley (Hordeum vulgare) seedlings was successfully established by utilizing high-speed counter-current chromatography (HSCCC). Separation was performed with a two-phase solvent system: ethyl acetate/n-butanol/water (3:2:5, v/v/v). A total of 24 mg lutonarin and 14 mg saponarin was obtained in one-step separation from 100 mg sample. High performance liquid chromatography (HPLC) analysis showed that the purity of each of the two compounds was over 98%. Their chemical structures were confirmed by UV, H-1 NMR, and C-13 NMR data.

引用

页码：2524 / 2532

页数：9

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